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Cytokeratin 17 Polyclonal antibody

Cytokeratin 17 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 22230-1-AP

Print datasheet

Synonyms

KRT17, Keratin-17, keratin 17, Cytokeratin-17, Cytokeratin,Keratin

验证数据展示

  • WB analysis of HeLa using 22230-1-AP

    WB analysis of HeLa using 22230-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 22230-1-AP (Cytokeratin 17 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 22230-1-AP

    IF Staining of HeLa using 22230-1-AP

    Immunofluorescent analysis of HeLa cells using 22230-1-AP (Cytokeratin 17 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human cervical cancer using 22230-1-AP

    IHC staining of human cervical cancer using 22230-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 22230-1-AP (Cytokeratin 17 antibody at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with KRT17 antibody included
  • IHC staining of human cervical cancer using 22230-1-AP

    IHC staining of human cervical cancer using 22230-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 22230-1-AP (Cytokeratin 17 antibody at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with KRT17 antibody included
  • IHC staining of human bowen disease using 22230-1-AP

    IHC staining of human bowen disease using 22230-1-AP

    Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 22230-1-AP (Cytokeratin 17 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KRT17 antibody included
  • IHC staining of human bowen disease using 22230-1-AP

    IHC staining of human bowen disease using 22230-1-AP

    Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 22230-1-AP (Cytokeratin 17 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KRT17 antibody included

经过测试的应用

Positive WB detected inHeLa cells, MCF-7 cells
Positive IHC detected inhuman bowen disease, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy KRT17 Ready-To-Use IHC Kit. KRT17 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22230-1-AP targets Cytokeratin 17 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen Cytokeratin 17 fusion protein Ag17575 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name keratin 17
Synonyms KRT17, Keratin-17, keratin 17, Cytokeratin-17, Cytokeratin,Keratin
Calculated Molecular Weight 432 aa, 48 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberBC000159
Gene Symbol Cytokeratin 17
Gene ID (NCBI) 3872
RRIDAB_2879040
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ04695
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 17 is a type I cytokeratin. It is found in nail beds, hair follicles, sebaceous glands, and other epidermal appendages. It is used as a marker for trauma.

实验方案

Product Specific Protocols
WB protocol for Cytokeratin 17 antibody 22230-1-APDownload protocol
IHC protocol for Cytokeratin 17 antibody 22230-1-APDownload protocol
IF protocol for Cytokeratin 17 antibody 22230-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols