Cytokeratin 2e Polyclonal antibody
Cytokeratin 2e Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | A431 cells, mouse skin tissue, rat skin tissue. |
Positive IHC detected in | human cervical cancer tissue, human oesophagus cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
IF | See 1 publications below |
产品信息
21725-1-AP targets Cytokeratin 2e in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, ELISA Application Description |
Cited Applications | IF |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human |
Immunogen | Cytokeratin 2e fusion protein Ag16384 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | keratin 2 |
Synonyms | CK 2e, Cytokeratin 2e, Epithelial keratin 2e, K2e, keratin 2, Keratin 2 epidermis, Keratin 2e, KRT2, KRT2A, KRT2E, KRTE, Type II keratin Kb2 |
Calculated Molecular Weight | 639 aa, 66 kDa |
Observed Molecular Weight | 65 kDa |
GenBank Accession Number | BC096294 |
Gene Symbol | KRT2 |
Gene ID (NCBI) | 3849 |
RRID | AB_2878914 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P35908 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for Cytokeratin 2e antibody 21725-1-AP | Download protocol |
IHC protocol for Cytokeratin 2e antibody 21725-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cont Lens Anterior Eye The biomechanical proteins different between low myopic corneas and moderate to high myopic corneas in human |