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KRT23 Polyclonal antibody

KRT23 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 24049-1-AP

Print datasheet

Synonyms

K23, Cytokeratin-23, Cytokeratin 23 , CK-23, CK23

验证数据展示

  • WB analysis of BxPC-3 using 24049-1-AP

    WB analysis of BxPC-3 using 24049-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 24049-1-AP (KRT23 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 24049-1-AP

    IHC staining of human colon cancer using 24049-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24049-1-AP (KRT23 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KRT23 antibody included
  • IHC staining of human colon cancer using 24049-1-AP

    IHC staining of human colon cancer using 24049-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24049-1-AP (KRT23 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KRT23 antibody included
  • IHC staining of human bowen disease using 24049-1-AP

    IHC staining of human bowen disease using 24049-1-AP

    Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 24049-1-AP (KRT23 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KRT23 antibody included
  • IHC staining of human bowen disease using 24049-1-AP

    IHC staining of human bowen disease using 24049-1-AP

    Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 24049-1-AP (KRT23 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KRT23 antibody included

经过测试的应用

Positive WB detected inBxPC-3 cells, HepG2 cells, HeLa cells
Positive IHC detected inhuman colon cancer tissue, human skin cancer tissue, human placenta tissue, human liver cancer tissue, rat colon tissue, mouse colon tissue, human bowen disease
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy KRT23 Ready-To-Use IHC Kit. KRT23 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

24049-1-AP targets KRT23 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsIF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen KRT23 fusion protein Ag21129 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name keratin 23 (histone deacetylase inducible)
Synonyms K23, Cytokeratin-23, Cytokeratin 23 , CK-23, CK23
Calculated Molecular Weight 422 aa, 48 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberBC028356
Gene Symbol KRT23
Gene ID (NCBI) 25984
RRIDAB_2879416
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9C075
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT23 belongs to type I keratins.

实验方案

Product Specific Protocols
WB protocol for KRT23 antibody 24049-1-APDownload protocol
IHC protocol for KRT23 antibody 24049-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Cell Rep

Delamination of trophoblast-like syncytia from the amniotic ectodermal analogue in human primed embryonic stem cell-based differentiation model.

Authors - Masatoshi Ohgushi
View Article