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验证数据展示

  • WB analysis of MCF-7 using 11048-1-AP

    WB analysis of MCF-7 using 11048-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 11048-1-AP (KRT33B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human skin cancer using 11048-1-AP

    IHC staining of human skin cancer using 11048-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 11048-1-AP (KRT33B antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KRT33B antibody included
  • IHC staining of human skin cancer using 11048-1-AP

    IHC staining of human skin cancer using 11048-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 11048-1-AP (KRT33B antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KRT33B antibody included
  • IF Staining of MCF-7 using 11048-1-AP

    IF Staining of MCF-7 using 11048-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 11048-1-AP (KRT33B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

KRT33B Polyclonal antibody

KRT33B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 11048-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

Keratin-33B, Keratin, type I cuticular Ha3-II, keratin 33B, K33B, HHA3-II

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMCF-7 cells, human brain tissue
Positive IHC detected inhuman skin cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy KRT33B Ready-To-Use IHC Kit. KRT33B primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

11048-1-AP targets KRT33B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原 KRT33B fusion protein Ag1522 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 keratin 33B
别名 Keratin-33B, Keratin, type I cuticular Ha3-II, keratin 33B, K33B, HHA3-II
计算分子量 46 kDa
观测分子量 46 kDa
GenBank蛋白编号BC009971
基因名称 KRT33B
Gene ID (NCBI) 3884
RRIDAB_2133874
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ14525
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 33 belongs to type I keratin and is found in hair and nails. There are two isoforms of this protein, encoded by two separate genes, KRTHA3A and KRTHA3B.

实验方案

Product Specific Protocols
WB protocol for KRT33B antibody 11048-1-APDownload protocol
IHC protocol for KRT33B antibody 11048-1-APDownload protocol
IF protocol for KRT33B antibody 11048-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Asian J Androl

PMID: 24994782

Authors - Yan-Min Ma
View Article