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KRT34 Polyclonal antibody

KRT34 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 20449-1-AP

Print datasheet

Synonyms

keratin 34, KRT 34, Keratin-34, keratin34

验证数据展示

  • WB analysis of HeLa using 20449-1-AP

    WB analysis of HeLa using 20449-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20449-1-AP (KRT34 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human skin cancer using 20449-1-AP

    IHC staining of human skin cancer using 20449-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 20449-1-AP (KRT34 antibody) at dilution of 1:200 (under 10x lens).

    Ready-to-use IHC kits available with KRT34 antibody included
  • IHC staining of human skin cancer using 20449-1-AP

    IHC staining of human skin cancer using 20449-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 20449-1-AP (KRT34 antibody) at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with KRT34 antibody included
  • IF Staining of HepG2 using 20449-1-AP

    IF Staining of HepG2 using 20449-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 20449-1-AP (KRT34 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, A375 cells, A431 cells, HepG2 cells, L02 cells, mouse skin tissue
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy KRT34 Ready-To-Use IHC Kit. KRT34 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20449-1-AP targets KRT34 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen KRT34 fusion protein Ag14295 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name keratin 34
Synonyms keratin 34, KRT 34, Keratin-34, keratin34
Calculated Molecular Weight 436 aa, 49 kDa
Observed Molecular Weight 49 kDa
GenBank Accession NumberBC041070
Gene Symbol KRT34
Gene ID (NCBI) 3885
RRIDAB_10700000
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO76011
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells. Keratin expression is highly regulated, tissue specific, and varies according to cell-state. Type I keratins consist of acidic, low molecular weight proteins with MW ranging from 40 kDa (KRT19) to 64 kDa (KRT9). Type 2 keratins consist of basic or neutral, high molecular weight proteins with MW from 52 kDa (KRT8) to 67 kDa (KRT18). KRT 34 is a type I keratin.

实验方案

Product Specific Protocols
WB protocol for KRT34 antibody 20449-1-APDownload protocol
IHC protocol for KRT34 antibody 20449-1-APDownload protocol
IF protocol for KRT34 antibody 20449-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols