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验证数据展示

  • WB analysis of HeLa using 20449-1-AP

    WB analysis of HeLa using 20449-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20449-1-AP (KRT34 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human skin cancer using 20449-1-AP

    IHC staining of human skin cancer using 20449-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 20449-1-AP (KRT34 antibody) at dilution of 1:200 (under 10x lens).

    Ready-to-use IHC kits available with KRT34 antibody included
  • IHC staining of human skin cancer using 20449-1-AP

    IHC staining of human skin cancer using 20449-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 20449-1-AP (KRT34 antibody) at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with KRT34 antibody included
  • IF Staining of HepG2 using 20449-1-AP

    IF Staining of HepG2 using 20449-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 20449-1-AP (KRT34 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Cytokeratin 34 Polyclonal antibody

Cytokeratin 34 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 20449-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

keratin 34, KRT34, keratin34, Keratin-34, KRT 34

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, A375 cells, A431 cells, HepG2 cells, L02 cells, mouse skin tissue
Positive IHC detected inhuman skin cancer tissue, human lung squamous cell carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy KRT34 Ready-To-Use IHC Kit. KRT34 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20449-1-AP targets Cytokeratin 34 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 Cytokeratin 34 fusion protein Ag14295 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 keratin 34
别名 keratin 34, KRT34, keratin34, Keratin-34, KRT 34
计算分子量 436 aa, 49 kDa
观测分子量 49 kDa
GenBank蛋白编号BC041070
基因名称 KRT34
Gene ID (NCBI) 3885
RRIDAB_10700000
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO76011
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells. Keratin expression is highly regulated, tissue specific, and varies according to cell-state. Type I keratins consist of acidic, low molecular weight proteins with MW ranging from 40 kDa (KRT19) to 64 kDa (KRT9). Type 2 keratins consist of basic or neutral, high molecular weight proteins with MW from 52 kDa (KRT8) to 67 kDa (KRT18). KRT 34 is a type I keratin.

实验方案

Product Specific Protocols
WB protocol for Cytokeratin 34 antibody 20449-1-APDownload protocol
IHC protocol for Cytokeratin 34 antibody 20449-1-APDownload protocol
IF protocol for Cytokeratin 34 antibody 20449-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols