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验证数据展示

  • WB analysis of A431 using 16853-1-AP

    WB analysis of A431 using 16853-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 16853-1-AP (Cytokeratin 6A-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human skin cancer using 16853-1-AP

    IHC staining of human skin cancer using 16853-1-AP

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 16853-1-AP (Cytokeratin 6A-specific antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KRT6 antibody included
  • IHC staining of human skin cancer using 16853-1-AP

    IHC staining of human skin cancer using 16853-1-AP

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 16853-1-AP (Cytokeratin 6A-specific antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KRT6 antibody included
  • IP experiment of HeLa using 16853-1-AP

    IP experiment of HeLa using 16853-1-AP

    IP result of anti-Cytokeratin 6A-specific (IP:16853-1-AP, 4ug; Detection:16853-1-AP 1:300) with HeLa cells lysate 520ug.

  • WB analysis of AML12 using 16853-1-AP

    WB analysis of AML12 using 16853-1-AP

    WB result of Cytokeratin 6A antibody (16853-1-AP; 1:600; room temperature for 1.5 hours) with wild-type and Cytokeratin 6A knockout AML 12 cells.

  • Featured Product
  • KD/KO验证

Cytokeratin 6A-specific Polyclonal antibody

Cytokeratin 6A-specific Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 16853-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IP, ELISA

别名

Cytokeratin 6, KRT6A, CK-6D, CK-6A, CK6A

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, AML12 cells, HeLa cells, human saliva, mouse skin tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman skin cancer tissue, human bowen disease
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy KRT6 Ready-To-Use IHC Kit. KRT6 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16853-1-AP targets Cytokeratin 6A-specific in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IP, ELISA Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 keratin 6A
别名 Cytokeratin 6, KRT6A, CK-6D, CK-6A, CK6A
计算分子量 60 kDa
观测分子量 56 kDa
GenBank蛋白编号NM_005554
基因名称 Cytokeratin 6A
Gene ID (NCBI) 3853
RRIDAB_2134304
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP02538
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 6 is a type II keratin. It is used as marker for epidermal hyperproliferation and differentiation. Three keratin 6 isoforms were found, ketatin 6A,6B and 6C. They share more than 99% identical DNA sequence. This antibody is against all three isoforms of keratin 6, but it does not bind other keratins.

实验方案

Product Specific Protocols
WB protocol for Cytokeratin 6A-specific antibody 16853-1-APDownload protocol
IHC protocol for Cytokeratin 6A-specific antibody 16853-1-APDownload protocol
IP protocol for Cytokeratin 6A-specific antibody 16853-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols