• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Cytokeratin 6A-specific Polyclonal antibody

Cytokeratin 6A-specific Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 16853-1-AP

Print datasheet

Synonyms

Cytokeratin 6, KRT6A, CK-6D, CK-6A, CK6A

验证数据展示

  • WB analysis of A431 using 16853-1-AP

    WB analysis of A431 using 16853-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 16853-1-AP (Cytokeratin 6A-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human skin cancer using 16853-1-AP

    IHC staining of human skin cancer using 16853-1-AP

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 16853-1-AP (Cytokeratin 6A-specific antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KRT6 antibody included
  • IHC staining of human skin cancer using 16853-1-AP

    IHC staining of human skin cancer using 16853-1-AP

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 16853-1-AP (Cytokeratin 6A-specific antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KRT6 antibody included
  • IP experiment of HeLa using 16853-1-AP

    IP experiment of HeLa using 16853-1-AP

    IP result of anti-Cytokeratin 6A-specific (IP:16853-1-AP, 4ug; Detection:16853-1-AP 1:300) with HeLa cells lysate 520ug.

经过测试的应用

Positive WB detected inA431 cells, mouse skin tissue, HeLa cells, human saliva tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman skin cancer tissue, human bowen disease
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy KRT6 Ready-To-Use IHC Kit. KRT6 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16853-1-AP targets Cytokeratin 6A-specific in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IP, ELISA Application Description
Tested Reactivity human, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name keratin 6A
Synonyms Cytokeratin 6, KRT6A, CK-6D, CK-6A, CK6A
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 56 kDa
GenBank Accession NumberNM_005554
Gene Symbol Cytokeratin 6A
Gene ID (NCBI) 3853
RRIDAB_2134304
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP02538
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 6 is a type II keratin. It is used as marker for epidermal hyperproliferation and differentiation. Three keratin 6 isoforms were found, ketatin 6A,6B and 6C. They share more than 99% identical DNA sequence. This antibody is against all three isoforms of keratin 6, but it does not bind other keratins.

实验方案

Product Specific Protocols
WB protocol for Cytokeratin 6A-specific antibody 16853-1-APDownload protocol
IHC protocol for Cytokeratin 6A-specific antibody 16853-1-APDownload protocol
IP protocol for Cytokeratin 6A-specific antibody 16853-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols