Cytokeratin 7 Polyclonal antibody
Cytokeratin 7 Polyclonal Antibody for WB, IF/ICC, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IF/ICC, IHC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | A431 cells, HeLa cells, HepG2 cells |
Positive IHC detected in | human lung cancer tissue, human pancreas tissue, human kidney tissue, human breast cancer tissue, human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | A549 cells, HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
Immunofluorescence (IF) | IF : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
IHC | See 1 publications below |
产品信息
22208-1-AP targets Cytokeratin 7 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.
Tested Applications | WB, IF/ICC, IHC, ELISA Application Description |
Cited Applications | IHC |
Tested Reactivity | human |
Cited Reactivity | human |
Immunogen | Cytokeratin 7 fusion protein Ag17528 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | keratin 7 |
Synonyms | Cell and organelle markers, CK 7, CK7, Cytokeratin 7, Cytoskeleton Marker, K2C7, K7, keratin 7, KRT7, Sarcolectin, SCL, Type II keratin Kb7 |
Calculated Molecular Weight | 469 aa, 51 kDa |
Observed Molecular Weight | 51 kDa |
GenBank Accession Number | BC002700 |
Gene Symbol | KRT7 |
Gene ID (NCBI) | 3855 |
RRID | AB_2879030 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P08729 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for Cytokeratin 7 antibody 22208-1-AP | Download protocol |
IHC protocol for Cytokeratin 7 antibody 22208-1-AP | Download protocol |
IF protocol for Cytokeratin 7 antibody 22208-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Ginekol Pol Expression profiles of human somatic mesenchymal stem cells derived from fresh endometrium, ectopic-endometrium and umbilical cord |