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Cytokeratin 7-specific Polyclonal antibody

Cytokeratin 7-specific Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16857-1-AP

Print datasheet

Synonyms

CK 7, CK7, Cytokeratin 7, Cytokeratin 7-specific, Cytoskeleton Marker, K2C7, K7, keratin 7, KRT7, Sarcolectin, SCL, Type II keratin Kb7

验证数据展示

  • IHC staining of human lung cancer using 16857-1-AP

    IHC staining of human lung cancer using 16857-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16857-1-AP (Cytokeratin 7-specific antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Cytokeratin 7 antibody included
  • IHC staining of human lung cancer using 16857-1-AP

    IHC staining of human lung cancer using 16857-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16857-1-AP (Cytokeratin 7-specific antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Cytokeratin 7 antibody included
  • WB analysis of HepG2 using 16857-1-AP

    WB analysis of HepG2 using 16857-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16857-1-AP (KRT7-specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHepG2 cells
Positive IHC detected inhuman lung cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy Cytokeratin 7 Ready-To-Use IHC Kit. Cytokeratin 7 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16857-1-AP targets Cytokeratin 7-specific in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name keratin 7
Synonyms CK 7, CK7, Cytokeratin 7, Cytokeratin 7-specific, Cytoskeleton Marker, K2C7, K7, keratin 7, KRT7, Sarcolectin, SCL, Type II keratin Kb7
Calculated Molecular Weight 51 kDa
Observed Molecular Weight 51 kDa
GenBank Accession NumberNM_005556
Gene Symbol Cytokeratin 7
Gene ID (NCBI) 3855
RRIDAB_2134446
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP08729
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT7, also named as cytokeratin 7, is one member of type II basic cytokeratin. It is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels, and their neoplasms. KRT7 is marker of epithelial tissues, but not present in carcinomas of stratified squamous cell origin.This antibody is specifically against KRT7.

实验方案

Product Specific Protocols
WB protocol for Cytokeratin 7-specific antibody 16857-1-APDownload protocol
IHC protocol for Cytokeratin 7-specific antibody 16857-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols