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Conjugate
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验证数据展示

  • WB analysis of HeLa using 83415-3-RR (same clone as 83415-3-PBS)

    WB analysis of HeLa using 83415-3-RR (same clone as 83415-3-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 83415-3-RR (KRT77 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83415-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83415-3-RR

    Affinity and Kinetic Characterization of 83415-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83415-3-RR against Human KRT77 were performed. The affinity constant is 0.209 nM.

Cytokeratin 77 Recombinant antibody, PBS Only

Cytokeratin 77 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 83415-3-PBS

产品说明书

CloneNo. 240490A11

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

KRT77, 240490A11, CK-1B, Cytokeratin,Keratin, Cytokeratin-1B

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83415-3-PBS targets Cytokeratin 77 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 Cytokeratin 77 fusion protein Ag21942 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 keratin 77
别名 KRT77, 240490A11, CK-1B, Cytokeratin,Keratin, Cytokeratin-1B
计算分子量 578 aa, 62 kDa
观测分子量61 kDa
GenBank蛋白编号BC122558
基因名称 KRT77
Gene ID (NCBI) 374454
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ7Z794
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into epithelial keratins and hair keratins.The KRT77 gene is a type II epithelial cell α-keratin gene family member that is expressed in the skin and eccrine sweat glands. KRT77 has N- and C-terminal domains and a central rod domain consisting of 4 alpha-helical regions separated by 3 nonhelical linker sequences.