验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, A549 cells, C6 cells, HeLa cells, MCF-7 cells |
| Positive IHC detected in | human stomach tissue, human colon, human bowen disease, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse testis tissue |
| Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17514-1-AP targets Cytokeratin 8 in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF-P, FC (Intra), ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, zebrafish, hamster |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | keratin 8 |
| 别名 | KRT8, Cytokeratin-8, Cytokeratin,Keratin, CK-8, CK8 |
| 计算分子量 | 54 kDa |
| 观测分子量 | 54 kDa |
| GenBank蛋白编号 | NM_002273 |
| 基因名称 | Cytokeratin 8 |
| Gene ID (NCBI) | 3856 |
| RRID | AB_2134726 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P05787 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT8 is often paired with keratin 18 in vivo. This antibody is specifically against KRT8.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Cytokeratin 8 antibody 17514-1-AP | Download protocol |
| IHC protocol for Cytokeratin 8 antibody 17514-1-AP | Download protocol |
| IF protocol for Cytokeratin 8 antibody 17514-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Oncogene The keratin 17/YAP/IL6 axis contributes to E-cadherin loss and aggressiveness of diffuse gastric cancer | ||
Clin Transl Med Aptamer-SH2 superbinder-based targeted therapy for pancreatic ductal adenocarcinoma. | ||
Sci Rep Conversion of embryonic stem cells into extraembryonic lineages by CRISPR-mediated activators. | ||
iScience Cysteine Dioxygenase Regulates the Epithelial Morphogenesis of Mammary Gland via Cysteine Sulfinic Acid. | ||
Regen Ther Dendrobium officinale polysaccharide promotes angiogenesis as well as follicle regeneration and hair growth through activation of the WNT signaling pathway | ||
Front Med (Lausanne) An Integrated Proteomics and Metabolomics Strategy for the Mechanism of Calcium Oxalate Crystal-Induced Kidney Injury. |
