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验证数据展示

  • WB analysis of HepG2 using 24409-1-AP

    WB analysis of HepG2 using 24409-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 24409-1-AP (KTELC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of C2C12 using 24409-1-AP

    IF Staining of C2C12 using 24409-1-AP

    Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using KTELC1 antibody (24409-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human liver using 24409-1-AP

    IHC staining of human liver using 24409-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 24409-1-AP (KTELC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

KTELC1 Polyclonal antibody

KTELC1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 24409-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

C3orf9, CAP10 like 46 kDa protein, CLP46, hCLP46, KDELCL1, KTELC1, MDS010, MDSRP, UNQ490/PRO1006

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, HEK-293 cells, Hela cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inC2C12 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24409-1-AP targets KTELC1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 KTELC1 fusion protein Ag19942 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 KTEL (Lys-Tyr-Glu-Leu) containing 1
别名 C3orf9, CAP10 like 46 kDa protein, CLP46, hCLP46, KDELCL1, KTELC1, MDS010, MDSRP, UNQ490/PRO1006
计算分子量 392 aa, 46 kDa
观测分子量 65 kDa
GenBank蛋白编号BC048810
基因名称 KTELC1
Gene ID (NCBI) 56983
RRIDAB_2879528
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8NBL1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KTELC1 also termed as CLP46 is a glucosyltransferase, which implicates in Protein modification and glycosylation. KTELC1 encodes a 392 amino acids protein and its molecular weight is 46kda, but is exists glycosylation modification. KTELC1 is localized in endoplasmic reticulum lumen and abundantly expressed in liver, brain, heart, skeletal muscle, spleen, kidney, placenta, lung and peripheral blood leukocyte. KTELC1 may regulation in cell proliferation and Notch signal pathway.1

实验方案

Product Specific Protocols
WB protocol for KTELC1 antibody 24409-1-APDownload protocol
IHC protocol for KTELC1 antibody 24409-1-APDownload protocol
IF protocol for KTELC1 antibody 24409-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols