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KTI12 Polyclonal antibody

KTI12 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28237-1-AP

Print datasheet

Synonyms

TOT4, SBBI81, KTI 12

验证数据展示

  • WB analysis using 28237-1-AP

    WB analysis using 28237-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28237-1-AP (KTI12 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human intrahepatic cholangiocarcinoma using 28237-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 28237-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 28237-1-AP (KTI12 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:8000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28237-1-AP targets KTI12 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen KTI12 fusion protein Ag28201 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name KTI12 homolog, chromatin associated (S. cerevisiae)
Synonyms TOT4, SBBI81, KTI 12
Calculated Molecular Weight 354 aa, 39 kDa
Observed Molecular Weight 39 kDa
GenBank Accession NumberBC012173
Gene Symbol KTI12
Gene ID (NCBI) 112970
RRIDAB_2881094
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96EK9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for KTI12 antibody 28237-1-APDownload protocol
IHC protocol for KTI12 antibody 28237-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols