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  • Featured Product
  • KD/KO Validated

Keratin 81 Polyclonal antibody, PBS Only

Keratin 81 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 11342-1-PBS

Print datasheet

Synonyms

验证数据展示

  • WB analysis using 11342-1-AP (same clone as 11342-1-PBS)

    WB analysis using 11342-1-AP (same clone as 11342-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11342-1-AP (KRT81 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11342-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 11342-1-AP (same clone as 11342-1-PBS)

    IHC staining of human colon cancer using 11342-1-AP (same clone as 11342-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 11342-1-AP (KRT81 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11342-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with KRT81 antibody included
  • IP experiment of HEK-293 using 11342-1-AP (same clone as 11342-1-PBS)

    IP experiment of HEK-293 using 11342-1-AP (same clone as 11342-1-PBS)

    IP result of anti-KRT81 (IP:11342-1-AP, 4ug; Detection:11342-1-AP 1:300) with HEK-293 cells lysate 2800ug. This data was developed using the same antibody clone with 11342-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 11342-1-AP (same clone as 11342-1-PBS)

    IF Staining of HeLa using 11342-1-AP (same clone as 11342-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using KRT81 antibody (11342-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11342-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy KRT81 Ready-To-Use IHC Kit. KRT81 primary antibody included.

产品信息

11342-1-PBS targets Keratin 81 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen Keratin 81 fusion protein Ag1890 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name keratin 81
Synonyms
Calculated Molecular Weight 55 kDa
Observed Molecular Weight 54 kDa
GenBank Accession NumberBC021241
Gene Symbol KRT81
Gene ID (NCBI) 3887
RRIDAB_2132771
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14533
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT81 is a type II keratin.