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Ki-67 Polyclonal antibody

Ki-67 Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 32270-1-AP

Print datasheet

Synonyms

MKI67, KI67, Proliferation marker protein Ki-67, Ki 67, Antigen KI-67

验证数据展示

  • IHC staining of human tonsillitis using 32270-1-AP

    IHC staining of human tonsillitis using 32270-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 32270-1-AP (Ki-67 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KI67 antibody included
  • IHC staining of human tonsillitis using 32270-1-AP

    IHC staining of human tonsillitis using 32270-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 32270-1-AP (Ki-67 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KI67 antibody included
  • IF Staining of HeLa using 32270-1-AP

    IF Staining of HeLa using 32270-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Ki-67 antibody (32270-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-phalloidin (green).

经过测试的应用

Positive IHC detected inhuman tonsillitis tissue, human colon cancer tissue, human ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy KI67 Ready-To-Use IHC Kit. KI67 primary antibody included.

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32270-1-AP targets Ki-67 in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name antigen identified by monoclonal antibody Ki-67
Synonyms MKI67, KI67, Proliferation marker protein Ki-67, Ki 67, Antigen KI-67
Calculated Molecular Weight 359 kDa
GenBank Accession NumberNM_002417
Gene Symbol KI67
Gene ID (NCBI) 4288
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDP46013
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation. Ki67 is present during all active phases of the cell cycle (G1, S, G2 and M), but is absent in resting cells (G0). Cellular content of Ki-67 protein markedly increases during cell progression through S phase of the cell cycle. Therefore, the nuclear expression of Ki67 can be evaluated to assess tumor proliferation by immunohistochemistry. It has been demonstrated to be of prognostic value in breast cancer. In head and neck cancer, several studies have reported an association between high proliferative activity and poorer prognosis.

实验方案

Product Specific Protocols
IHC protocol for Ki-67 antibody 32270-1-APDownload protocol
IF protocol for Ki-67 antibody 32270-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols