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Kindlin 2 Polyclonal antibody

Kindlin 2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29911-1-AP

Print datasheet

Synonyms

FERMT2, FERMT 2, mig 2, Kindlin-2, kindlin2

验证数据展示

  • WB analysis using 29911-1-AP

    WB analysis using 29911-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29911-1-AP (Kindlin 2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IHC staining of human normal colon using 29911-1-AP

    IHC staining of human normal colon using 29911-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 29911-1-AP (Kindlin 2 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FERMT2/Kindlin 2 antibody included
  • IHC staining of human normal colon using 29911-1-AP

    IHC staining of human normal colon using 29911-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 29911-1-AP (Kindlin 2 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FERMT2/Kindlin 2 antibody included
  • IHC staining of human lung cancer using 29911-1-AP

    IHC staining of human lung cancer using 29911-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 29911-1-AP (Kindlin 2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FERMT2/Kindlin 2 antibody included

经过测试的应用

Positive WB detected inA549 cells, HEK-293 cells, HeLa cells
Positive IHC detected inhuman lung cancer tissue, human normal colon
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy FERMT2/Kindlin 2 Ready-To-Use IHC Kit. FERMT2/Kindlin 2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29911-1-AP targets Kindlin 2 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen Kindlin 2 fusion protein Ag31795 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name fermitin family homolog 2 (Drosophila)
Synonyms FERMT2, FERMT 2, mig 2, Kindlin-2, kindlin2
Calculated Molecular Weight 680 aa, 78 kDa
Observed Molecular Weight78 kDa
GenBank Accession NumberBC017327
Gene Symbol Kindlin 2
Gene ID (NCBI) 10979
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96AC1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FERMT2, also named as KIND2, MIG2, PLEKHC1, MIG-2 and Kindlin-2, belongs to the kindlin family. There are three FERMT2 isoforms that participate in the connection between extracellular matrix (ECM) adhesion sites and the actin cytoskeleton and also in the orchestration of actin assembly and cell shape modulation. FERMT2 recruits migfilin (FBLP1) protein to cell-ECM focal adhesion sites. Variable expression in human cancers and mesenchymal cancer invasion are now linked with FERMT2, which may plays important role in carcinormas development. Catalog#11453-1-AP is a rabbit polyclonal antibody against human FERMT2.

实验方案

Product Specific Protocols
WB protocol for Kindlin 2 antibody 29911-1-APDownload protocol
IHC protocol for Kindlin 2 antibody 29911-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols