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验证数据展示

  • WB analysis using 29911-1-AP

    WB analysis using 29911-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29911-1-AP (Kindlin 2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 29911-1-AP

    IHC staining of human colon using 29911-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 29911-1-AP (Kindlin 2 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FERMT2/Kindlin 2 antibody included
  • IHC staining of human colon using 29911-1-AP

    IHC staining of human colon using 29911-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 29911-1-AP (Kindlin 2 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FERMT2/Kindlin 2 antibody included
  • IHC staining of human lung cancer using 29911-1-AP

    IHC staining of human lung cancer using 29911-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 29911-1-AP (Kindlin 2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FERMT2/Kindlin 2 antibody included

Kindlin 2 Polyclonal antibody

Kindlin 2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 29911-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

FERMT2, FERMT 2, mig 2, Kindlin-2, kindlin2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, HEK-293 cells, HeLa cells
Positive IHC detected inhuman lung cancer tissue, human normal colon
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy FERMT2/Kindlin 2 Ready-To-Use IHC Kit. FERMT2/Kindlin 2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29911-1-AP targets Kindlin 2 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 Kindlin 2 fusion protein Ag31795 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 fermitin family homolog 2 (Drosophila)
别名 FERMT2, FERMT 2, mig 2, Kindlin-2, kindlin2
计算分子量 680 aa, 78 kDa
观测分子量78 kDa
GenBank蛋白编号BC017327
基因名称 Kindlin 2
Gene ID (NCBI) 10979
RRIDAB_3086186
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96AC1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FERMT2, also named as KIND2, MIG2, PLEKHC1, MIG-2 and Kindlin-2, belongs to the kindlin family. There are three FERMT2 isoforms that participate in the connection between extracellular matrix (ECM) adhesion sites and the actin cytoskeleton and also in the orchestration of actin assembly and cell shape modulation. FERMT2 recruits migfilin (FBLP1) protein to cell-ECM focal adhesion sites. Variable expression in human cancers and mesenchymal cancer invasion are now linked with FERMT2, which may plays important role in carcinormas development.

实验方案

Product Specific Protocols
WB protocol for Kindlin 2 antibody 29911-1-APDownload protocol
IHC protocol for Kindlin 2 antibody 29911-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols