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Kininogen 1 Polyclonal antibody

Kininogen 1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27477-1-AP

Print datasheet

Synonyms

BDK, Fitzgerald factor, HMWK, Ile Ser Bradykinin Bradykinin, Kallidin I Lysyl bradykinin, kininogen 1, KNG, KNG1

验证数据展示

  • WB analysis using 27477-1-AP

    WB analysis using 27477-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27477-1-AP (Kininogen 1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 27477-1-AP

    IHC staining of human kidney using 27477-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27477-1-AP (Kininogen 1 antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human kidney using 27477-1-AP

    IHC staining of human kidney using 27477-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27477-1-AP (Kininogen 1 antibody) at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive WB detected inA2780 cells, HeLa cells, HepG2 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27477-1-AP targets Kininogen 1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen Kininogen 1 fusion protein Ag26467 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name kininogen 1
Synonyms BDK, Fitzgerald factor, HMWK, Ile Ser Bradykinin Bradykinin, Kallidin I Lysyl bradykinin, kininogen 1, KNG, KNG1
Calculated Molecular Weight 644 aa, 72 kDa
Observed Molecular Weight 70-72 kDa
GenBank Accession NumberBC060039
Gene Symbol Kininogen 1
Gene ID (NCBI) 3827
RRIDAB_2880882
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP01042
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Kininogens are inhibitors of thiol proteases. Kininogen 1 plays important role in Kinin-kallikrein system. This gene is translated into High-molecular weight kininogen (HMWK) and low-molecular weight kininogen (LMWK) after alternative splicing. HMWK is produced by the liver together with prekallikrein. It acts mainly as a cofactor on coagulation and inflammation, and has no intrinsic catalytic activity. LMWK is produced locally by numerous tissues, and secreted together with tissue kallikrein.

实验方案

Product Specific Protocols
WB protocol for Kininogen 1 antibody 27477-1-APDownload protocol
IHC protocol for Kininogen 1 antibody 27477-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols