mouse brain tissues (heated at 100°C, unboided, heated at 37°C) were subjected to SDS PAGE followed by western blot with 12503-1-AP (Kir4.1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12503-1-PBS in a different storage buffer formulation.

IP result of anti-Kir4.1 (IP:12503-1-AP, 4ug; Detection:12503-1-AP 1:500) with rat brain tissue lysate 4800ug. This data was developed using the same antibody clone with 12503-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12503-1-AP (Kir4.1 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12503-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12503-1-AP (Kir4.1 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12503-1-PBS in a different storage buffer formulation.