• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 85133-3-RR

    WB analysis using 85133-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85133-3-RR (KCND2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of DU 145 using 85133-3-RR

    WB analysis of DU 145 using 85133-3-RR

    DU 145 cells were subjected to SDS PAGE followed by western blot with 85133-3-RR (KCND2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse cerebellum using 85133-3-RR

    IHC staining of mouse cerebellum using 85133-3-RR

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 85133-3-RR (Kv4.2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse brain using 85133-3-RR

    IF Staining of mouse brain using 85133-3-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using Kv4.2 antibody (85133-3-RR, Clone: 242754F4 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 85133-3-RR

    Affinity and Kinetic Characterization of 85133-3-RR

    Biolayer interferometry (BLl) kinetic assays of 85133-3-RR against Human Kv4.2 were performed. The affinity constant is below 1 pM.

Kv4.2 Recombinant antibody

Kv4.2 Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 85133-3-RR

产品说明书

CloneNo. 242754F4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF-P, ELISA

别名

KCND2, 242754F4, KIAA1044, Potassium voltage-gated channel subfamily D member 2, RK5

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHeLa cells, DU 145 cells, A549 cells
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85133-3-RR targets Kv4.2 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse
免疫原 Kv4.2 fusion protein Ag15879 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 potassium voltage-gated channel, Shal-related subfamily, member 2
别名 KCND2, 242754F4, KIAA1044, Potassium voltage-gated channel subfamily D member 2, RK5
计算分子量 630 aa, 71 kDa
观测分子量75 kDa
GenBank蛋白编号BC110449
基因名称 KCND2
Gene ID (NCBI) 3751
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9NZV8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Voltage-gated potassium or Kv channels, specifically those mediating low threshold, rapidly inactivating Ito and IA currents, are known to regulate cardiac and neuronal membrane excitability, respectively (PMID: 12829703). Voltage-gated potassium channel subunit Kv4.2, encoded by the KCND2 gene, belongs to the potassium channel family and D (Shal) subfamily. It is a pore-forming alpha subunit of voltage-gated rapidly inactivating A-type potassium channels. Kv4.2 is highly expressed in the brain (PMID: 10551270). It is a major constituent of A-type potassium currents and a key regulator of neuronal membrane excitability (PMID: 22539834).

实验方案

Product Specific Protocols
WB protocol for Kv4.2 antibody 85133-3-RRDownload protocol
IHC protocol for Kv4.2 antibody 85133-3-RRDownload protocol
IF protocol for Kv4.2 antibody 85133-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols