验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, K-562 cells, Ramos cells |
Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | A431 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:2000-1:16000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
55208-1-AP targets L-Plastin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
经测试反应性 | human, mouse |
免疫原 | Peptide 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | lymphocyte cytosolic protein 1 (L-plastin) |
别名 | L PLASTIN, LCP 1, LCP1, LPL, L-Plastin, Lymphocyte cytosolic protein 1, Plastin 2, PLS2 |
计算分子量 | 70 kDa |
观测分子量 | 64-70 kDa |
GenBank蛋白编号 | NM_002298 |
基因名称 | L-Plastin |
Gene ID (NCBI) | 3936 |
RRID | AB_2881288 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P13796 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for L-Plastin antibody 55208-1-AP | Download protocol |
IHC protocol for L-Plastin antibody 55208-1-AP | Download protocol |
IF protocol for L-Plastin antibody 55208-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |