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验证数据展示

  • IF Staining of A431 using 55208-1-AP

    IF Staining of A431 using 55208-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed A431 cells using 55208-1-AP (L-Plastin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • WB analysis using 55208-1-AP

    WB analysis using 55208-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 55208-1-AP (L-Plastin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 55208-1-AP

    IHC staining of human colon cancer using 55208-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55208-1-AP (L-Plastin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

L-Plastin Polyclonal antibody

L-Plastin Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 55208-1-AP

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

L PLASTIN, LCP 1, LCP1, LPL, L-Plastin, Lymphocyte cytosolic protein 1, Plastin 2, PLS2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, K-562 cells, Ramos cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55208-1-AP targets L-Plastin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name lymphocyte cytosolic protein 1 (L-plastin)
Synonyms L PLASTIN, LCP 1, LCP1, LPL, L-Plastin, Lymphocyte cytosolic protein 1, Plastin 2, PLS2
Calculated Molecular Weight 70 kDa
Observed Molecular Weight 64-70 kDa
GenBank Accession NumberNM_002298
Gene Symbol L-Plastin
Gene ID (NCBI) 3936
RRIDAB_2881288
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP13796
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for L-Plastin antibody 55208-1-APDownload protocol
IHC protocol for L-Plastin antibody 55208-1-APDownload protocol
IF protocol for L-Plastin antibody 55208-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols