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L1CAM Polyclonal antibody, PBS Only

L1CAM Polyclonal Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

Cat no : 20659-1-PBS

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Synonyms

验证数据展示

  • WB analysis using 20659-1-AP (same clone as 20659-1-PBS)

    WB analysis using 20659-1-AP (same clone as 20659-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20659-1-AP (L1CAM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20659-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy L1CAM Ready-To-Use IHC Kit. L1CAM primary antibody included.

产品信息

20659-1-PBS targets L1CAM in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name L1 cell adhesion molecule
Synonyms
Calculated Molecular Weight 140 kDa
Observed Molecular Weight 200-220 kDa, 80 kDa
GenBank Accession NumberNM_000425
Gene Symbol L1CAM
Gene ID (NCBI) 3897
RRIDAB_2878717
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP32004
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

L1CAM, also known as NCAM-L1 or CD171, is a cell adhesion molecule of the immunoglobulin superfamily. It is a 200-220 kDa transmembrane glycoprotein composed of six Ig-like domains and five fibronectin type III repeats followed by a transmembrane region and a highly conserved cytoplasmic tail (PMID: 3412448; 22796939). L1CAM is primarily expressed in the nervous system and is involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, cerebellar granule cell migration, neurite outgrowth on Schwann cells and interactions among epithelial cells of intestinal crypts (PMID: 3412448; 10767310). L1CAM is overexpressed in many human cancers and is often associated with bad prognosis (PMID: 27267927; 26111503). It has been reported that L1CAM can be proteolytically cleaved into a soluble fragment of 140 kDa and an intracellular fragment of 80-85 kDa (PMID: 30842511; 34380733). This antibody raised against 1206-1221aa of human L1CAM detects 200-220 kDa full-length L1CAM and 80-kDa cleaved fragment of L1CAM.