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L1CAM Monoclonal antibody, PBS Only

L1CAM Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Pig, Mouse, Rat, Rabbit

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3H7B9

Cat no : 67115-1-PBS

Print datasheet

Synonyms

CAML1, CD171, HSAS, HSAS1, L1 cell adhesion molecule, L1CAM, MASA, MIC5, N CAM L1, N CAML1, NCAM L1, S10, SPG1

验证数据展示

  • WB analysis using 67115-1-Ig (same clone as 67115-1-PBS)

    WB analysis using 67115-1-Ig (same clone as 67115-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67115-1-Ig (L1CAM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67115-1-PBS in a different storage buffer formulation.

  • IHC staining of human brain using 67115-1-Ig (same clone as 67115-1-PBS)

    IHC staining of human brain using 67115-1-Ig (same clone as 67115-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 67115-1-Ig (L1CAM antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67115-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with L1CAM antibody included
  • IHC staining of human brain using 67115-1-Ig (same clone as 67115-1-PBS)

    IHC staining of human brain using 67115-1-Ig (same clone as 67115-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 67115-1-Ig (L1CAM antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67115-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with L1CAM antibody included
  • IF Staining of mouse brain using 67115-1-Ig (same clone as 67115-1-PBS)

    IF Staining of mouse brain using 67115-1-Ig (same clone as 67115-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using L1CAM antibody (67115-1-Ig, Clone: 3H7B9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67115-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 67115-1-Ig (same clone as 67115-1-PBS)

    IF Staining of mouse brain using 67115-1-Ig (same clone as 67115-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using L1CAM antibody (67115-1-Ig, Clone: 3H7B9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67115-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy L1CAM Ready-To-Use IHC Kit. L1CAM primary antibody included.

产品信息

67115-1-PBS targets L1CAM in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with Human, Pig, Mouse, Rat, Rabbit samples.

Tested Applications WB, IHC, IF-P, Indirect ELISA Application Description
Tested Reactivity Human, Pig, Mouse, Rat, Rabbit
Immunogen L1CAM fusion protein Ag17706 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name L1 cell adhesion molecule
Synonyms CAML1, CD171, HSAS, HSAS1, L1 cell adhesion molecule, L1CAM, MASA, MIC5, N CAM L1, N CAML1, NCAM L1, S10, SPG1
Calculated Molecular Weight 1257 aa, 140 kDa
Observed Molecular Weight 220 kDa
GenBank Accession NumberBC126229
Gene Symbol L1CAM
Gene ID (NCBI) 3897
RRIDAB_2882419
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP32004
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

L1CAM, also known as NCAM-L1 or CD171, is a cell adhesion molecule of the immunoglobulin superfamily. It is a 200-220 kDa transmembrane glycoprotein composed of six Ig-like domains and five fibronectin type III repeats followed by a transmembrane region and a highly conserved cytoplasmic tail (PMID: 3412448; 22796939). L1CAM is primarily expressed in the nervous system and is involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, cerebellar granule cell migration, neurite outgrowth on Schwann cells and interactions among epithelial cells of intestinal crypts (PMID: 3412448; 10767310). L1CAM is overexpressed in many human cancers and is often associated with bad prognosis (PMID: 27267927; 26111503).