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Anti-Human L1CAM/CD171 Rabbit Recombinant Antibody, PBS Only

L1CAM/CD171 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

240978A7

Cat no : 98130-1-PBS

Print datasheet

Synonyms

L1CAM, L1 cell adhesion molecule, HSAS1, HSAS, CD171

验证数据展示

  • FC experiment of SK-N-SH using 98130-1-RR (same clone as 98130-1-PBS)

    FC experiment of SK-N-SH using 98130-1-RR (same clone as 98130-1-PBS)

    1x10^6 SK-N-SH cells were surface stained with 0.25 ug Anti-Human L1CAM/CD171 Rabbit Recombinant Antibody (98130-1-RR, Clone:240978A7) or 0.25 ug Isotype Control, and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005). Cells were not fixed. This data was developed using the same antibody clone with 98130-1-PBS in a different storage buffer formulation.

  • FC experiment of SK-N-SH using 98130-1-RR (same clone as 98130-1-PBS)

    FC experiment of SK-N-SH using 98130-1-RR (same clone as 98130-1-PBS)

    1x10^6 SK-N-SH cells were surface stained with 0.25 ug Anti-Human L1CAM/CD171 Rabbit Recombinant Antibody (98130-1-RR, Clone: 240978A7) (red) or Isotype Control (blue), and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005). Cells were not fixed. This data was developed using the same antibody clone with 98130-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 98130-1-RR

    Affinity and Kinetic Characterization of 98130-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98130-1-RR against Human L1CAM/CD171 were performed. The affinity constant is 0.243 nM.

Planning an IHC experiment? We recommend our IHCeasy L1CAM Ready-To-Use IHC Kit. L1CAM primary antibody included.

产品信息

The immunogen of 98130-1-PBS is L1CAM/CD171 Fusion Protein expressed in E. coli.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name L1 cell adhesion molecule
Synonyms L1CAM, L1 cell adhesion molecule, HSAS1, HSAS, CD171
Calculated Molecular Weight 1257 aa, 140 kDa
GenBank Accession NumberBC126229
Gene Symbol L1CAM
Gene ID (NCBI) 3897
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP32004
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

L1CAM, also known as NCAM-L1 or CD171, is a cell adhesion molecule of the immunoglobulin superfamily. It is a 200-220 kDa transmembrane glycoprotein composed of six Ig-like domains and five fibronectin type III repeats followed by a transmembrane region and a highly conserved cytoplasmic tail (PMID: 3412448; 22796939). L1CAM is primarily expressed in the nervous system and is involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, cerebellar granule cell migration, neurite outgrowth on Schwann cells and interactions among epithelial cells of intestinal crypts (PMID: 3412448; 10767310). L1CAM is overexpressed in many human cancers and is often associated with bad prognosis (PMID: 27267927; 26111503). It has been reported that L1CAM can be proteolytically cleaved into a soluble fragment of 140 kDa and an intracellular fragment of 80-85 kDa (PMID: 30842511; 34380733). This antibody raised against 1206-1221aa of human L1CAM detects 200-220 kDa full-length L1CAM and 80-kDa cleaved fragment of L1CAM.