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L1CAM Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human

Detection: human

Applications

Sandwich ELISA

Range

Sandwich ELISA: 62.5-4000 pg/mL

Cat No : MP00954-3

Print datasheet

Synonyms

Validation Data Gallery

  • Sandwich ELISA standard curve of MP00954-3, Human L1CAM Recombinant Matched Antibody Pair - PBS only. 84034-6-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0209. 84034-3-PBS was HRP conjugated as the detection antibody. Range: 62.5-4000 pg/mL.

    Sandwich ELISA standard curve of MP00954-3

    Sandwich ELISA standard curve of MP00954-3, Human L1CAM Recombinant Matched Antibody Pair - PBS only. 84034-6-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0209. 84034-3-PBS was HRP conjugated as the detection antibody. Range: 62.5-4000 pg/mL.

  • Serum of eight individual healthy human donors was measured. The L1CAM concentration of detected samples was determined to be 8,620.0 pg/mL with a range of 2,796.0 - 11,053.6 pg/mL.

    Sample test of MP00954-3

    Serum of eight individual healthy human donors was measured. The L1CAM concentration of detected samples was determined to be 8,620.0 pg/mL with a range of 2,796.0 - 11,053.6 pg/mL.

  • The mean L1CAM concentration was determined to be 185.4 ng/mL in HeLa cell extract based on a 3.6 mg/mL extract load.

    Sample test of MP00954-3

    The mean L1CAM concentration was determined to be 185.4 ng/mL in HeLa cell extract based on a 3.6 mg/mL extract load.

Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

MP00954-3 targets L1CAM in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 62.5-4000 pg/mL (Sandwich ELISA);

Capture antibody: L1CAM Recombinant antibody, PBS Only (Capture) 84034-6-PBS (240978B10). 100 μg. Concentration 1 mgl/ml.

Detection antibody: L1CAM Recombinant antibody, PBS Only (Detector) 84034-3-PBS (240978G3). 100 μg. Concentration 1 mgl/ml.

Alternative L1CAM matched antibody pairs: MP00954-1, MP00954-2, MP50443-1

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 84034-6-PBS
Tested Reactivity human
Immunogen Fusion Protein
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name L1 cell adhesion molecule
Synonyms L1 cell adhesion molecule, HSAS1, HSAS, CD171, CAML1
GenBank accession numberBC126229
Gene symbol L1CAM
UniprotP32004
Gene ID (NCBI) 3897
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
Catalog Number 84034-3-PBS
Tested Reactivity human
Immunogen Fusion Protein
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name L1 cell adhesion molecule
Synonyms L1 cell adhesion molecule, HSAS1, HSAS, CD171, CAML1
GenBank accession numberBC126229
Gene symbol L1CAM
UniprotP32004
Gene ID (NCBI) 3897
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C