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验证数据展示

  • WB analysis of NCCIT cells using 21528-1-AP

    WB analysis of NCCIT cells using 21528-1-AP

    WB result of L1TD1 antibody (21528-1-AP, 1:500) with NCCIT cells.

  • IHC staining of human ovary cancer using 21528-1-AP

    IHC staining of human ovary cancer using 21528-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 21528-1-AP (L1TD1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of NCCIT using 21528-1-AP

    IF Staining of NCCIT using 21528-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed NCCIT cells using L1TD1 antibody (21528-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

L1TD1 Polyclonal antibody

L1TD1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 21528-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

LINE-1 type transposase domain-containing protein 1, ES cell-associated protein 11, ES cell associated protein 11, ECAT11

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inNCCIT cells
Positive IHC detected inhuman ovary cancer tissue, human ovary tumor tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNCCIT cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21528-1-AP targets L1TD1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 L1TD1 fusion protein Ag16147 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 LINE-1 type transposase domain containing 1
别名 LINE-1 type transposase domain-containing protein 1, ES cell-associated protein 11, ES cell associated protein 11, ECAT11
计算分子量 865 aa, 99 kDa
观测分子量 100 kDa
GenBank蛋白编号BC111559
基因名称 L1TD1
Gene ID (NCBI) 54596
RRIDAB_2878878
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5T7N2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

L1TD1 is an RNA-binding protein highly and specifically expressed in pluripotent cells. It is essential for maintaining the pluripotentency in human embryonic stem cells (hESCs) and has been considered as a marker for undifferentiated hESCs. L1TD1 is also reported to be highly expressed in medulloblastoma cells and its expression in medulloblastoma correlates with poor clinical outcome.

实验方案

Product Specific Protocols
IHC protocol for L1TD1 antibody 21528-1-APDownload protocol
IF protocol for L1TD1 antibody 21528-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols