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验证数据展示

  • WB analysis using 28112-1-AP

    WB analysis using 28112-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28112-1-AP (L3MBTL3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse cerebellum using 28112-1-AP

    IHC staining of mouse cerebellum using 28112-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 28112-1-AP (L3MBTL3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of U2OS using 28112-1-AP

    IF Staining of U2OS using 28112-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using L3MBTL3 antibody (28112-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

L3MBTL3 Polyclonal antibody

L3MBTL3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 28112-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, Mouse

应用

WB, IHC, IF/ICC, ELISA

别名

H l(3)mbt like protein 3, KIAA1798, l(3)mbt like 3 (Drosophila), L(3)mbt like protein 3, L3MBTL3, MBT 1, MBT1, RP11 73O6.1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293T cells, HeLa cells, Jurkat cells
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28112-1-AP targets L3MBTL3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 Human, Mouse
免疫原 L3MBTL3 fusion protein Ag27136 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 l(3)mbt-like 3 (Drosophila)
别名 H l(3)mbt like protein 3, KIAA1798, l(3)mbt like 3 (Drosophila), L(3)mbt like protein 3, L3MBTL3, MBT 1, MBT1, RP11 73O6.1
计算分子量 88 aa, 3 kDa
观测分子量88 kDa
GenBank蛋白编号BC060845
基因名称 L3MBTL3
Gene ID (NCBI) 84456
RRIDAB_2918148
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96JM7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

L3MBTL3, also named as MBT1 and KIAA1798, belongs to the Malignant brain tumor (MBT) family. L3MBTL3 can bind to methyl lysine in histone, further affecting the transcription and its abnormal regulation associated with many diseases (PMID: 34373992). Additionally, researches have shown that L3MBTL3 plays a role in meduloblastoma formation and normal hematopoiesis in humans (PMID: 26317848). L3MBTL3 has 2 isoforms with the molecular mass of 86 and 88 kDa.

实验方案

Product Specific Protocols
WB protocol for L3MBTL3 antibody 28112-1-APDownload protocol
IHC protocol for L3MBTL3 antibody 28112-1-APDownload protocol
IF protocol for L3MBTL3 antibody 28112-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols