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Species Reactivity
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验证数据展示

  • IF Staining of A549 using 66785-1-Ig (same clone as 66785-1-PBS)

    IF Staining of A549 using 66785-1-Ig (same clone as 66785-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using LACTB antibody (66785-1-Ig, Clone: 3C3C3 ) at dilution of 1:1600 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 66785-1-PBS in a different storage buffer formulation.

  • WB analysis using 66785-1-Ig (same clone as 66785-1-PBS)

    WB analysis using 66785-1-Ig (same clone as 66785-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66785-1-Ig (LACTB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66785-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66785-1-Ig (same clone as 66785-1-PBS)

    IHC staining of human liver cancer using 66785-1-Ig (same clone as 66785-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66785-1-Ig (LACTB antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66785-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66785-1-Ig (same clone as 66785-1-PBS)

    IHC staining of human liver cancer using 66785-1-Ig (same clone as 66785-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66785-1-Ig (LACTB antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66785-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66785-1-Ig (same clone as 66785-1-PBS)

    IF Staining of HepG2 using 66785-1-Ig (same clone as 66785-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66785-1-Ig (LACTB antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66785-1-PBS in a different storage buffer formulation.

LACTB Monoclonal antibody, PBS Only

LACTB Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66785-1-PBS

产品说明书

CloneNo. 3C3C3

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

Serine beta-lactamase-like protein LACTB, mitochondrial, MRPL56, lactamase, beta, G24, EC:3.4.-.-

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66785-1-PBS targets LACTB in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 LACTB fusion protein Ag12233 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 lactamase, beta
别名 Serine beta-lactamase-like protein LACTB, mitochondrial, MRPL56, lactamase, beta, G24, EC:3.4.-.-
计算分子量 547 aa, 61 kDa
观测分子量 50-60 kDa
GenBank蛋白编号BC067288
基因名称 LACTB
Gene ID (NCBI) 114294
RRIDAB_2882130
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP83111
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

LACTB is also named as MRPL56,whic is a component of the large subunit of the mitochondrial ribosome that is encoded by the nuclear genome.LACTB is a mammalian active-site serine protein that has evolved from a bacterial penicillin-binding protein.It promotes intramitochondrial membrane organization and micro-compartmentalization through polymerization(PMID:19858488). Northern blot analysis of mouse tissues detected expression at high levels in liver, heart, and skeletal muscle, with lower levels in kidney, testis, brain, lung, and spleen(PMID:11707067). The full-length of LACTB is about 61kDa, and a 115aa transit peptide can be removed in the mature form.