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  • Featured Product
  • KD/KO Validated

LACTB2 Polyclonal antibody

LACTB2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 16783-1-AP

Print datasheet

Synonyms

Endoribonuclease LACTB2, EC:3.1.27.-, CGI-83, CGI83, CGI 83

验证数据展示

  • WB analysis of A549 using 16783-1-AP

    WB analysis of A549 using 16783-1-AP

    WB result of LACTB2 antibody (16783-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LACTB2 transfected A549 cells.

  • WB analysis using 16783-1-AP

    WB analysis using 16783-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16783-1-AP (LACTB2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human cervix using 16783-1-AP

    IHC staining of human cervix using 16783-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervix using 16783-1-AP (LACTB2 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human cervix using 16783-1-AP

    IHC staining of human cervix using 16783-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervix using 16783-1-AP (LACTB2 antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of HepG2 using 16783-1-AP

    IF Staining of HepG2 using 16783-1-AP

    Immunofluorescent analysis of HepG2 cells, using LACTB2 antibody 16783-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • IP experiment of A549 using 16783-1-AP

    IP experiment of A549 using 16783-1-AP

    IP result of anti-LACTB2 (IP:16783-1-AP, 3ug; Detection:16783-1-AP 1:700) with A549 cells lysate 520ug.

经过测试的应用

Positive WB detected inMCF-7 cells, PC-3 cells, Y79 cells, A549 cells, HeLa cells
Positive IP detected inA549 cells
Positive IHC detected inhuman cervix tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16783-1-AP targets LACTB2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen LACTB2 fusion protein Ag10213 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name lactamase, beta 2
Synonyms Endoribonuclease LACTB2, EC:3.1.27.-, CGI-83, CGI83, CGI 83
Calculated Molecular Weight 288 aa, 33 kDa
Observed Molecular Weight 33 kDa
GenBank Accession NumberBC000878
Gene Symbol LACTB2
Gene ID (NCBI) 51110
RRIDAB_2133333
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ53H82
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for LACTB2 antibody 16783-1-APDownload protocol
IHC protocol for LACTB2 antibody 16783-1-APDownload protocol
IF protocol for LACTB2 antibody 16783-1-APDownload protocol
IP protocol for LACTB2 antibody 16783-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols