验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16136-1-AP targets LAD1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB, IF, IHC |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Immunogen | LAD1 fusion protein Ag9159 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | ladinin 1 |
| Synonyms | LAD, Lad 1, LAD1, LadA, ladinin 1, Linear IgA disease antigen |
| Calculated Molecular Weight | 517 aa, 57 kDa |
| Observed Molecular Weight | 57 kDa |
| GenBank Accession Number | BC009742 |
| Gene Symbol | LAD1 |
| Gene ID (NCBI) | 3898 |
| RRID | AB_2081705 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O00515 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Ladinin-1, also named as LADA, or LAD1, is an anchoring filament protein which is a component of the basement membrane zone. It is a novel component of the basement membranes and may function in contributing to the stability of the association of the epithelial layers with the underlying mesenchyme. Human ladinin is an autoantigen associated with linear IgA disease. It is a secreted protein. Immunohistochemical staining of 16136-1-AP shows strong extracellular matrixc positivity in human testis. The predicted MW of this protein is 57 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for LAD1 antibody 16136-1-AP | Download protocol |
| IHC protocol for LAD1 antibody 16136-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
