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LAG-3/CD223 Recombinant antibody, PBS Only

LAG-3/CD223 Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F14

Cat no : 80867-1-PBS

Print datasheet

Synonyms

LAG-3, CD223, 1F14, LAG3, LAG 3

验证数据展示

  • WB analysis of CTLL-2 using 80867-1-RR (same clone as 80867-1-PBS)

    WB analysis of CTLL-2 using 80867-1-RR (same clone as 80867-1-PBS)

    CTLL-2 cells were subjected to SDS PAGE followed by western blot with 80867-1-RR (LAG-3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80867-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 80867-1-RR

    Affinity and Kinetic Characterization of 80867-1-RR

    Biolayer interferometry (BLl) kinetic assays of 80867-1-RR against Human LAG-3/CD223 were performed. The affinity constant is 8.47 nM.

产品信息

80867-1-PBS targets LAG-3/CD223 in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen fusion protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name lymphocyte-activation gene 3
Synonyms LAG-3, CD223, 1F14, LAG3, LAG 3
Calculated Molecular Weight57 kDa
Observed Molecular Weight60 kDa
GenBank Accession NumberNM_002286
Gene Symbol LAG-3
Gene ID (NCBI) 3902
RRIDAB_2918917
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP18627
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

LAG-3, also known as CD223, is an immune checkpoint molecule that regulates both T-cell activation and homeostasis. LAG-3 is expressed on activated T cells, NK cells, regulatory T cells, and plasmacytoid dendritic cells. It is a CD4-related molecule that binds MHC class II. LAG-3 plays an important role in modulating T cell expansion and function, and blockade of LAG-3 with monoclonal antibodies can augment T cell function. (PMID: 15634887; 21086108; 28783703)