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LAG-3/CD223 Polyclonal antibody

LAG-3/CD223 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 31722-1-AP

Print datasheet

Synonyms

CD223, LAG-3, lymphocyte activation gene 3, Ly66, Lag3

验证数据展示

  • WB analysis using 31722-1-AP

    WB analysis using 31722-1-AP

    Untreated or Con A treated mouse splenocytes were subjected to SDS PAGE followed by western blot with 31722-1-AP (LAG-3/CD223 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inCon A treated mouse splenocytes

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31722-1-AP targets LAG-3/CD223 in WB, ELISA applications and shows reactivity with mouse samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity mouse
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name lymphocyte-activation gene 3
Synonyms CD223, LAG-3, lymphocyte activation gene 3, Ly66, Lag3
Calculated Molecular Weight57kDa
Observed Molecular Weight65-75 kDa
GenBank Accession NumberNM_008479.2
Gene Symbol CD223
Gene ID (NCBI) 16768
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ61790
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LAG-3, also known as CD223, is an immune checkpoint molecule that regulates both T-cell activation and homeostasis. LAG-3 is expressed on activated T cells, NK cells, regulatory T cells, and plasmacytoid dendritic cells. It is a CD4-related molecule that binds MHC class II. LAG-3 plays an important role in modulating T cell expansion and function, and blockade of LAG-3 with monoclonal antibodies can augment T cell function. (PMID: 15634887; 21086108; 28783703)

实验方案

Product Specific Protocols
WB protocol for LAG-3/CD223 antibody 31722-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols