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LAG-3/CD223 Polyclonal antibody

LAG-3/CD223 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat No : 29548-1-AP

Print datasheet

Synonyms

LAG-3, CD223, lymphocyte activation gene 3, LAG3, LAG 3

验证数据展示

  • IHC staining of human tonsillitis using 29548-1-AP

    IHC staining of human tonsillitis using 29548-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 29548-1-AP (LAG-3 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 29548-1-AP

    IHC staining of human tonsillitis using 29548-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 29548-1-AP (LAG-3 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 2 publications below

产品信息

29548-1-AP targets LAG-3/CD223 in IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, ELISA Application Description
Cited ApplicationsIHC
Tested Reactivity human
Cited Reactivityhuman
Immunogen fusion protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name lymphocyte-activation gene 3
Synonyms LAG-3, CD223, lymphocyte activation gene 3, LAG3, LAG 3
Calculated Molecular Weight57 kDa
GenBank Accession NumberNM_002286
Gene Symbol LAG-3
Gene ID (NCBI) 3902
RRIDAB_2918324
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP18627
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LAG-3, also known as CD223, is an immune checkpoint molecule that regulates both T-cell activation and homeostasis. LAG-3 is expressed on activated T cells, NK cells, regulatory T cells, and plasmacytoid dendritic cells. It is a CD4-related molecule that binds MHC class II. LAG-3 plays an important role in modulating T cell expansion and function, and blockade of LAG-3 with monoclonal antibodies can augment T cell function. (PMID: 15634887; 21086108; 28783703)

实验方案

Product Specific Protocols
IHC protocol for LAG-3/CD223 antibody 29548-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Front Immunol

Prognostic value and immune landscapes of TERT promoter methylation in triple negative breast cancer

Authors - Fei Lin
View Article
humanIHC

Eur J Med Res

A combined analysis of multi-omics data reveals the prognostic values and immunotherapy response of LAG3 in human cancers

Authors - Jinwu Peng
View Article