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验证数据展示

  • IF Staining of THP-1 using 84677-4-RR

    IF Staining of THP-1 using 84677-4-RR

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using LAIR1 antibody (84677-4-RR, Clone: 241805C2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IHC staining of human tonsillitis using 84677-4-RR

    IHC staining of human tonsillitis using 84677-4-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84677-4-RR (LAIR1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 84677-4-RR

    IHC staining of human tonsillitis using 84677-4-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84677-4-RR (LAIR1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

LAIR1 Recombinant antibody

LAIR1 Recombinant Antibody for IHC, IF/ICC, ELISA
Cat No. 84677-4-RR

产品说明书

CloneNo. 241805C2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF/ICC, ELISA

别名

Leukocyte-associated immunoglobulin-like receptor 1, LAIR-1, LAIR 1, hLAIR1, CD305

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inTHP-1 cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84677-4-RR targets LAIR1 in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 leukocyte-associated immunoglobulin-like receptor 1
别名 Leukocyte-associated immunoglobulin-like receptor 1, LAIR-1, LAIR 1, hLAIR1, CD305
计算分子量 287 aa, 31 kDa
GenBank蛋白编号BC027899
基因名称 LAIR1
Gene ID (NCBI) 3903
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ6GTX8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Leukocyte-associated immunoglobulin-like receptor 1 (LAIR1) is a transmembrane glycoprotein with a single immunoglobulin-like domain and a cytoplasmic tail containing two immune receptor tyrosine-based inhibitory motifs (PMID: 9285412). LAIR1 is expressed on the majority of human PBMCs, including NK, T, B, monocytes, and dendritic cells, as well as the majority of thymocytes (PMID: 10229813). It functions as an inhibitory receptor that plays a constitutive negative regulatory role on cytolytic function of NK cells, B cells and T cells. LAIR1 is a collagen receptor (PMID: 16754721). Tumor-expressed collagens can modulate immune cell function through the inhibitory collagen receptor LAIR1 (PMID: 21955987).

实验方案

Product Specific Protocols
IHC protocol for LAIR1 antibody 84677-4-RRDownload protocol
IF protocol for LAIR1 antibody 84677-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols