• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 28697-1-AP

    WB analysis using 28697-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28697-1-AP (LAMA1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IF Staining of rat colon using 28697-1-AP

    IF Staining of rat colon using 28697-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat colon tissue using LAMA1 antibody (28697-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

LAMA1 Polyclonal antibody

LAMA1 Polyclonal Antibody for WB, IF-P, ELISA
Cat No. 28697-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IF-P, ELISA

别名

Laminin 1 subunit alpha, laminin, alpha 1, LAMA, Laminin subunit alpha-1, Laminin subunit alpha 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHeLa cells, U2OS cells
Positive IF-P detected inrat colon tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28697-1-AP targets LAMA1 in WB, IF-P, ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IF-P, ELISA Application Description
经测试反应性 human, rat
免疫原 LAMA1 fusion protein Ag29329 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 laminin, alpha 1
别名 Laminin 1 subunit alpha, laminin, alpha 1, LAMA, Laminin subunit alpha-1, Laminin subunit alpha 1
计算分子量 337 kDa
观测分子量337 kDa
GenBank蛋白编号NM_005559
基因名称 LAMA1
Gene ID (NCBI) 284217
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP25391
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LAMA1 is also known as laminin subunit α1. Laminins are a family of glycoproteins found in the extracellular matrix that comprises the basement membrane. Laminins have a heterotrimeric structure composed of an α, β, and γ chain. Numerous biological processes are known to be directed by them, including cell adhesion, mitogenesis, differentiation, and metastasis, all of which contribute to carcinogenesis. Tissue distribution of LAMA1 occurred mainly in early epithelial development and some adult epithelia. Recent reports have shown that laminin-1 acts as an efficient attachment protein for a large variety of cultured cell types in vitro. (PMID: 36824663)

实验方案

Product Specific Protocols
WB protocol for LAMA1 antibody 28697-1-APDownload protocol
IF protocol for LAMA1 antibody 28697-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols