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LAMA3 Recombinant antibody, PBS Only (Detector)

LAMA3 Uni-rAbTM Recombinant Antibody for WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

230443C1

Cat no : 83210-2-PBS

Print datasheet

Synonyms

LAMNA, Laminin subunit alpha 3, lama3a, E170, BM600

验证数据展示

  • Cytometric bead array standard curve of MP00186-2

    Cytometric bead array standard curve of MP00186-2

    Cytometric bead array standard curve of MP00186-2, LAMA3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83210-1-PBS. Detection antibody: 83210-2-PBS. Standard: SY01122. Range: 0.156-10 ng/mL.

  • Sandwich ELISA standard curve of MP00186-2

    Sandwich ELISA standard curve of MP00186-2

    Sandwich ELISA standard curve of MP00186-2, Human LAMA3 Recombinant Matched Antibody Pair - PBS only. 83210-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard SY01122. 83210-2-PBS was HRP conjugated as the detection antibody. Range: 0.131-20 ng/mL.

  • Sample test of MP00186-2

    Sample test of MP00186-2

    The mean LAMA3 concentration was determined to be 27.50 ng/mL in HeLa cell extract based on a 3.60 mg/mL extract load, 51.60 ng/mL in HepG2 cell extract based on a 5.00 mg/mL extract load and 21.80 ng/mL in A431 cell extract based on a 2.60 mg/mL extract load.

  • WB analysis using 83210-2-RR (same clone as 83210-2-PBS)

    WB analysis using 83210-2-RR (same clone as 83210-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83210-2-RR (LAMA3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83210-2-PBS in a different storage buffer formulation.

  • IHC staining of human intrahepatic cholangiocarcinoma using 83210-2-RR (same clone as 83210-2-PBS)

    IHC staining of human intrahepatic cholangiocarcinoma using 83210-2-RR (same clone as 83210-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83210-2-RR (LAMA3 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83210-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00186-3

    Cytometric bead array standard curve of MP00186-3

    Cytometric bead array standard curve of MP00186-3, LAMA3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83210-4-PBS. Detection antibody: 83210-2-PBS. Standard: SY01122. Range: 0.156-5 ng/mL.

产品信息

83210-2-PBS targets LAMA3 as part of a matched antibody pair:

MP00186-2: 83210-1-PBS capture and 83210-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

MP00186-3: 83210-4-PBS capture and 83210-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name laminin, alpha 3
Synonyms LAMNA, Laminin subunit alpha 3, lama3a, E170, BM600
Calculated Molecular Weight 367 kDa
Observed Molecular Weight260 kDa
GenBank Accession NumberNM_000227
Gene Symbol LAMA3
Gene ID (NCBI) 3909
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ16787
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Laminin subunit alpha 3 (LAMA3) expression is mediated by an epithelial enhancer via a synergistic effect of AP-1 binding sites.10 LAMA3 has been implicated in the invasion and progression of tumor cells and encodes for the α3 chain of laminin-5 (α3, β3, γ2, 332). Its dysfunction manifests as a blistering skin disease similar to junctional epidermolysis bullosa in humans. (PMID: 31557618)