• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • Cytometric bead array standard curve of MP00186-2

    Cytometric bead array standard curve of MP00186-2

    Cytometric bead array standard curve of MP00186-2, LAMA3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83210-1-PBS. Detection antibody: 83210-2-PBS. Standard: SY01122. Range: 0.156-10 ng/mL.

  • Sandwich ELISA standard curve of MP00186-2

    Sandwich ELISA standard curve of MP00186-2

    Sandwich ELISA standard curve of MP00186-2, Human LAMA3 Recombinant Matched Antibody Pair - PBS only. 83210-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard SY01122. 83210-2-PBS was HRP conjugated as the detection antibody. Range: 0.131-20 ng/mL.

  • Sample test of MP00186-2

    Sample test of MP00186-2

    The mean LAMA3 concentration was determined to be 27.50 ng/mL in HeLa cell extract based on a 3.60 mg/mL extract load, 51.60 ng/mL in HepG2 cell extract based on a 5.00 mg/mL extract load and 21.80 ng/mL in A431 cell extract based on a 2.60 mg/mL extract load.

  • WB analysis using 83210-2-RR (same clone as 83210-2-PBS)

    WB analysis using 83210-2-RR (same clone as 83210-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83210-2-RR (LAMA3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83210-2-PBS in a different storage buffer formulation.

  • IHC staining of human intrahepatic cholangiocarcinoma using 83210-2-RR (same clone as 83210-2-PBS)

    IHC staining of human intrahepatic cholangiocarcinoma using 83210-2-RR (same clone as 83210-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83210-2-RR (LAMA3 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83210-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00186-3

    Cytometric bead array standard curve of MP00186-3

    Cytometric bead array standard curve of MP00186-3, LAMA3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83210-4-PBS. Detection antibody: 83210-2-PBS. Standard: SY01122. Range: 0.156-5 ng/mL.

LAMA3 Recombinant antibody, PBS Only (Detector)

LAMA3 Uni-rAbTM Recombinant Antibody for WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 83210-2-PBS

产品说明书

CloneNo. 230443C1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

230443C1, BM600, E170, lama3a, Laminin subunit alpha 3

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

产品信息

83210-2-PBS targets LAMA3 as part of a matched antibody pair:

MP00186-2: 83210-1-PBS capture and 83210-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

MP00186-3: 83210-4-PBS capture and 83210-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 laminin, alpha 3
别名 230443C1, BM600, E170, lama3a, Laminin subunit alpha 3
计算分子量 367 kDa
观测分子量260 kDa
GenBank蛋白编号NM_000227
基因名称 LAMA3
Gene ID (NCBI) 3909
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ16787
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Laminin subunit alpha 3 (LAMA3) expression is mediated by an epithelial enhancer via a synergistic effect of AP-1 binding sites.10 LAMA3 has been implicated in the invasion and progression of tumor cells and encodes for the α3 chain of laminin-5 (α3, β3, γ2, 332). Its dysfunction manifests as a blistering skin disease similar to junctional epidermolysis bullosa in humans. (PMID: 31557618)