LAMA4 (Isoform 3) Polyclonal antibody

LAMA4 (Isoform 3) Polyclonal Antibody for WB, IHC, ELISA
Cat No. 10465-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IHC, ELISA

LAMA4 (Isoform 3), Laminin 14 subunit alpha, Laminin 8 subunit alpha, Laminin 9 subunit alpha, Laminin subunit alpha 4, laminin, alpha 4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inhuman heart tissue, human skeletal muscle tissue
Positive IHC detected inhuman ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10465-1-AP targets LAMA4 (Isoform 3) in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human
文献引用反应性human, mouse
免疫原 LAMA4 (Isoform 3) fusion protein Ag0736 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 laminin, alpha 4
别名 LAMA4 (Isoform 3), Laminin 14 subunit alpha, Laminin 8 subunit alpha, Laminin 9 subunit alpha, Laminin subunit alpha 4, laminin, alpha 4
计算分子量 13 kDa
观测分子量 11-13 kDa
GenBank蛋白编号BC004241
基因名称 Laminin alpha 4/LAMA4
Gene ID (NCBI) 3910
RRIDAB_2234461
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ16363
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Laminin is a basement membrane glycoprotein composed of three nonidentical chains, A, B1, and B2 (PMID: 7959779). LAMA4 (laminin, alpha 4), an A chain variant, is a subunit of laminin-8 (laminin-411), laminin-9 (laminin-421) and laminin-14 (laminin-423) (PMID: 10842354; 10964957). LAMA4 is widely distributed in developing and adult human tissues, and mainly localized to mesenchyme-derived tissues (PMID: 12133914). It may have a role in formation and function of endothelium, transmigration of inflammatory cells through endothelium, and invasion of certain tumors (PMID: 17533363). Three alternatively spliced mRNAs give rise to three isoforms. This antibody was generated against the full length (1-120aa) of isoform 3.

实验方案

Product Specific Protocols
WB protocol for LAMA4 (Isoform 3) antibody 10465-1-APDownload protocol
IHC protocol for LAMA4 (Isoform 3) antibody 10465-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Arthritis Rheum

PMID: 21337318

Authors - Gutierrez-Roelens I I
humanIHC

Sci Rep

Novel candidate factors predicting the effect of S-1 adjuvant chemotherapy of pancreatic cancer.

Authors - Katsutaka Mitachi
mouseIHC

Am J Physiol Heart Circ Physiol

Pygo1 regulates pathological cardiac hypertrophy via a β-catenin-dependent mechanism.

Authors - Li Lin
humanIHC

Neuromuscul Disord

Basement membrane remodelling and segmental fibrosis in sporadic inclusion body myositis.

Authors - Doppler K K
humanIHC

J Neurosci Res

Maturational changes in laminin, fibronectin, collagen IV, and perlecan in germinal matrix, cortex, and white matter and effect of betamethasone.

Authors - Xu Hongmin H
humanWB,IHC

Cancer Cell Int

MiR-539 inhibits proliferation and migration of triple-negative breast cancer cells by down-regulating LAMA4 expression.

Authors - Zhi-Xue Yang
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