LAMA4 (Isoform 3) Polyclonal antibody

LAMA4 (Isoform 3) Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10465-1-AP

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Synonyms

LAMA4 (Isoform 3), Laminin 14 subunit alpha, Laminin 8 subunit alpha, Laminin 9 subunit alpha, Laminin subunit alpha 4, laminin, alpha 4



经过测试的应用

Positive WB detected inhuman heart tissue, human skeletal muscle tissue
Positive IHC detected inhuman ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10465-1-AP targets LAMA4 (Isoform 3) in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen LAMA4 (Isoform 3) fusion protein Ag0736 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name laminin, alpha 4
Synonyms LAMA4 (Isoform 3), Laminin 14 subunit alpha, Laminin 8 subunit alpha, Laminin 9 subunit alpha, Laminin subunit alpha 4, laminin, alpha 4
Calculated Molecular Weight 13 kDa
Observed Molecular Weight 11-13 kDa
GenBank Accession NumberBC004241
Gene Symbol Laminin alpha 4/LAMA4
Gene ID (NCBI) 3910
RRIDAB_2234461
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ16363
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Laminin is a basement membrane glycoprotein composed of three nonidentical chains, A, B1, and B2 (PMID: 7959779). LAMA4 (laminin, alpha 4), an A chain variant, is a subunit of laminin-8 (laminin-411), laminin-9 (laminin-421) and laminin-14 (laminin-423) (PMID: 10842354; 10964957). LAMA4 is widely distributed in developing and adult human tissues, and mainly localized to mesenchyme-derived tissues (PMID: 12133914). It may have a role in formation and function of endothelium, transmigration of inflammatory cells through endothelium, and invasion of certain tumors (PMID: 17533363). Three alternatively spliced mRNAs give rise to three isoforms. This antibody was generated against the full length (1-120aa) of isoform 3.

实验方案

Product Specific Protocols
WB protocol for LAMA4 (Isoform 3) antibody 10465-1-APDownload protocol
IHC protocol for LAMA4 (Isoform 3) antibody 10465-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Arthritis Rheum

PMID: 21337318

Authors - Gutierrez-Roelens I I
humanIHC

Sci Rep

Novel candidate factors predicting the effect of S-1 adjuvant chemotherapy of pancreatic cancer.

Authors - Katsutaka Mitachi
mouseIHC

Am J Physiol Heart Circ Physiol

Pygo1 regulates pathological cardiac hypertrophy via a β-catenin-dependent mechanism.

Authors - Li Lin
humanIHC

Neuromuscul Disord

Basement membrane remodelling and segmental fibrosis in sporadic inclusion body myositis.

Authors - Doppler K K
humanIHC

J Neurosci Res

Maturational changes in laminin, fibronectin, collagen IV, and perlecan in germinal matrix, cortex, and white matter and effect of betamethasone.

Authors - Xu Hongmin H
humanWB,IHC

Cancer Cell Int

MiR-539 inhibits proliferation and migration of triple-negative breast cancer cells by down-regulating LAMA4 expression.

Authors - Zhi-Xue Yang