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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 82946-1-RR (same clone as 82946-1-PBS)

    WB analysis using 82946-1-RR (same clone as 82946-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82946-1-RR (LAMB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82946-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 82946-1-RR (same clone as 82946-1-PBS)

    IHC staining of human stomach cancer using 82946-1-RR (same clone as 82946-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 82946-1-RR (LAMB2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9). This data was developed using the same antibody clone with 82946-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 82946-1-RR (same clone as 82946-1-PBS)

    FC experiment of HepG2 using 82946-1-RR (same clone as 82946-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug LAMB2 Recombinant antibody (82946-1-RR, Clone:230143E4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82946-1-PBS in a different storage buffer formulation.

LAMB2 Recombinant antibody, PBS Only

LAMB2 Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), Indirect ELISA
Cat No. 82946-1-PBS

Print datasheet

CloneNo. 230143E4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC (Intra), Indirect ELISA

Synonyms

LAMS, Laminin 3 subunit beta, Laminin 15 subunit beta, Laminin 14 subunit beta, Laminin 11 subunit beta

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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产品信息

82946-1-PBS targets LAMB2 in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human
Immunogen LAMB2 fusion protein Ag33468 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name laminin, beta 2 (laminin S)
Synonyms LAMS, Laminin 3 subunit beta, Laminin 15 subunit beta, Laminin 14 subunit beta, Laminin 11 subunit beta
Calculated Molecular Weight196 kDa
Observed Molecular Weight195-200 kDa
GenBank Accession NumberNM_002292.3
Gene Symbol LAMB2
Gene ID (NCBI) 3913
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP55268
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

LAMB2 (laminin subunit beta 2), also known as LAMS. Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins, composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively), form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. This gene encodes the beta chain isoform laminin, beta 2. It is enriched in the basement membrane of muscles at the neuromuscular junctions, kidney glomerulus and vascular smooth muscle. Transgenic mice in which the beta 2 chain gene was inactivated by homologous recombination, showed defects in the maturation of neuromuscular junctions and impairment of glomerular filtration. The calculated molecular weight of LAMB2 is 200 kDa.