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验证数据展示

  • WB analysis using 82946-1-RR (same clone as 82946-1-PBS)

    WB analysis using 82946-1-RR (same clone as 82946-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82946-1-RR (LAMB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82946-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 82946-1-RR (same clone as 82946-1-PBS)

    IHC staining of human stomach cancer using 82946-1-RR (same clone as 82946-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 82946-1-RR (LAMB2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9). This data was developed using the same antibody clone with 82946-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 82946-1-RR (same clone as 82946-1-PBS)

    FC experiment of HepG2 using 82946-1-RR (same clone as 82946-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug LAMB2 Recombinant antibody (82946-1-RR, Clone:230143E4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82946-1-PBS in a different storage buffer formulation.

LAMB2 Recombinant antibody, PBS Only

LAMB2 Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), Indirect ELISA
Cat No. 82946-1-PBS

产品说明书

CloneNo. 230143E4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, FC (Intra), Indirect ELISA

别名

LAMS, Laminin 3 subunit beta, Laminin 15 subunit beta, Laminin 14 subunit beta, Laminin 11 subunit beta

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

82946-1-PBS targets LAMB2 in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原 LAMB2 fusion protein Ag33468 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 laminin, beta 2 (laminin S)
别名 LAMS, Laminin 3 subunit beta, Laminin 15 subunit beta, Laminin 14 subunit beta, Laminin 11 subunit beta
计算分子量196 kDa
观测分子量195-200 kDa
GenBank蛋白编号NM_002292.3
基因名称 LAMB2
Gene ID (NCBI) 3913
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP55268
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

LAMB2 (laminin subunit beta 2), also known as LAMS. Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins, composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively), form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. This gene encodes the beta chain isoform laminin, beta 2. It is enriched in the basement membrane of muscles at the neuromuscular junctions, kidney glomerulus and vascular smooth muscle. Transgenic mice in which the beta 2 chain gene was inactivated by homologous recombination, showed defects in the maturation of neuromuscular junctions and impairment of glomerular filtration. The calculated molecular weight of LAMB2 is 200 kDa.