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CD107b / LAMP2 Monoclonal antibody

CD107b / LAMP2 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66301-1-Ig

产品说明书

CloneNo. 2D3B9

宿主/亚型

Mouse / IgA

种属反应性

human, mouse, pig and More (2)

应用

WB, IHC, ELISA and More (2)

CD107b, LAMP2, LAMP-2, LAMP 2, CD107 antigen-like family member B

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inL02 cells, pig liver tissue, mouse liver tissue, HEK-293 cells, HUVEC cells, Jurkat cells, HeLa cells, HepG2 cells
Positive IHC detected inhuman liver cancer tissue, mouse liver tissue, human lung cancer tissue, human liver tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

This antibody is not recommended for immunocytofluorescent assays. Mouse monoclonal antibodies of IgA isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.

Planning an IHC experiment? We recommend our IHCeasy LAMP2 Ready-To-Use IHC Kit. LAMP2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66301-1-Ig targets CD107b / LAMP2 in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, pig samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC, IP, CoIP
经测试反应性 human, mouse, pig
文献引用反应性human, mouse, pig, chicken, bovine
免疫原 CD107b / LAMP2 fusion protein Ag0595 种属同源性预测
宿主/亚型 Mouse / IgA
抗体类别 Monoclonal
产品类型 Antibody
全称 lysosomal-associated membrane protein 2
别名 CD107b, LAMP2, LAMP-2, LAMP 2, CD107 antigen-like family member B
计算分子量 45 kDa
观测分子量 120 kDa
GenBank蛋白编号BC002965
基因名称 LAMP2
Gene ID (NCBI) 3920
ENSEMBL Gene IDENSG00000005893
RRIDAB_2881684
偶联类型 Unconjugated
形式 Liquid
纯化方式Thiophilic affinity chromatograph
UNIPROT IDP13473
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Lysosomal-associated membrane protein 2 (LAMP2, synonyms: LAMPB, CD107b) is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may play a role in tumor cell metastasis. It may also function in the protection, maintenance, and adhesion of the lysosome. Prior to posttranslational modification, LAMP2 is a ~45 kDa polypeptide. Mature, functional LAMP2 is extensively glycosylated with a variety of different N-linked and O-linked oligosaccharides.

实验方案

Product Specific Protocols
WB protocol for CD107b / LAMP2 antibody 66301-1-IgDownload protocol
IHC protocol for CD107b / LAMP2 antibody 66301-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Nature

Inhibition of PCSK9 potentiates immune checkpoint therapy for cancer.

Authors - Xinjian Liu
humanWB

Mol Cell

Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis.

Authors - Bin Jiang
humanIF

Autophagy

SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes

Authors - Wang Zheng
WB

Arthritis Rheumatol

LAMP3 induces lysosome-dependent cell death by impairing autophagic caspase-8 degradation in Sjögren's salivary glands

Authors - Hiroyuki Nakamura
humanIF

J Clin Invest

TMEM25 modulates neuronal excitability and NMDA receptor subunit NR2B degradation.

Authors - Haiqing Zhang
mouseWB

Redox Biol

WBP2 restrains the lysosomal degradation of GPX4 to inhibit ferroptosis in cisplatin-induced acute kidney injury

Authors - Zebin Deng
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