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LAMTOR1 Polyclonal antibody

LAMTOR1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 29611-1-AP

Print datasheet

Synonyms

C11orf59, p27RF-Rho, p27RF Rho, p27Kip1-releasing factor from RhoA, Lipid raft adaptor protein p18

验证数据展示

  • WB analysis using 29611-1-AP

    WB analysis using 29611-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29611-1-AP (C11orf59,LAMTOR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of U-87 MG using 29611-1-AP

    IF Staining of U-87 MG using 29611-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U-87 MG cells using LAMTOR1 antibody (29611-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

经过测试的应用

Positive WB detected inA431 cells, HEK-293 cells, MCF-7 cells
Positive IF/ICC detected inU-87 MG cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29611-1-AP targets LAMTOR1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen LAMTOR1 fusion protein Ag31215 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 11 open reading frame 59
Synonyms C11orf59, p27RF-Rho, p27RF Rho, p27Kip1-releasing factor from RhoA, Lipid raft adaptor protein p18
Calculated Molecular Weight 161 aa, 18 kDa
Observed Molecular Weight 18 kDa
GenBank Accession NumberBC001706
Gene Symbol LAMTOR1
Gene ID (NCBI) 55004
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6IAA8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LAMTOR1 (Late Endosome/Lysosome-Associated Membrane Protein 1) is a membrane protein specifically localized to the surface of late endosomes/lysosomes and is involved in lysosomal transport and maturation. LAMTOR1 is a key component of the lysosomal articulating protein complex Ragulator, which is required for the localization of the Ragulator complex to lysosomes. LAMTOR1 is a key component of the lysosomal bridging protein complex Ragulator and is required for the localization of the Ragulator complex to the lysosome. LAMTOR1 is involved in autophagy, cell growth, immune regulation, cancer development, and regulatory pathways of glucose metabolism. Recent studies have shown that in calorie restriction, lithocholic acid activates AMPK through the glucose-sensing pathway ( the lysosomal pathway ) and the body senses the metabolic signals induced by calorie restriction and exerts its role in slowing down the effects of aging and prolonging the lifespan, whereas deletion of LAMTOR1 results in the inability of AMPK to be activated in order to play its role.

实验方案

Product Specific Protocols
WB protocol for LAMTOR1 antibody 29611-1-APDownload protocol
IF protocol for LAMTOR1 antibody 29611-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols