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LAP3 Monoclonal antibody, PBS Only

LAP3 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B11B8

Cat no : 66417-1-PBS

Print datasheet

Synonyms

Cytosol aminopeptidase, LAP, LAP 3, LAP3, LAPEP, leucine aminopeptidase 3, Leucyl aminopeptidase, PEPS, Peptidase S, Proline aminopeptidase, Prolyl aminopeptidase

验证数据展示

  • IF Staining of HeLa using 66417-1-Ig (same clone as 66417-1-PBS)

    IF Staining of HeLa using 66417-1-Ig (same clone as 66417-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using LAP3 antibody (66417-1-Ig, Clone: 1B11B8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66417-1-PBS in a different storage buffer formulation.

  • WB analysis of NIH/3T3 using 66417-1-Ig (same clone as 66417-1-PBS)

    WB analysis of NIH/3T3 using 66417-1-Ig (same clone as 66417-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66417-1-Ig (LAP3 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66417-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung using 66417-1-Ig (same clone as 66417-1-PBS)

    IHC staining of human lung using 66417-1-Ig (same clone as 66417-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 66417-1-Ig (LAP3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66417-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung using 66417-1-Ig (same clone as 66417-1-PBS)

    IHC staining of human lung using 66417-1-Ig (same clone as 66417-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 66417-1-Ig (LAP3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66417-1-PBS in a different storage buffer formulation.

产品信息

66417-1-PBS targets LAP3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen LAP3 fusion protein Ag6300 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name leucine aminopeptidase 3
Synonyms Cytosol aminopeptidase, LAP, LAP 3, LAP3, LAPEP, leucine aminopeptidase 3, Leucyl aminopeptidase, PEPS, Peptidase S, Proline aminopeptidase, Prolyl aminopeptidase
Calculated Molecular Weight 56 kDa
Observed Molecular Weight 56 kDa
GenBank Accession NumberBC065564
Gene Symbol LAP3
Gene ID (NCBI) 51056
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP28838
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

LAP3(Leucine aminopeptidase 3) is also named as LAPEP, PEPS(peptidase S) and belongs to the peptidase M17 family. It can catalyzes the removal of unsubstituted N-terminal amino-acids from various peptides and be presumably involved in the processing end regular turnover of intracellular proteins. PEPS is found in a wide variety of tissues and cultured cells but not in red cells and skin. The enzyme can utilize several di-, tri-, and tetrapeptides as substrates and is the slowest migrating of the peptidases after electrophoresis.(PMID:689684). LAP3 has two isoform with MW of 52 and 56 kDa.