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LAPTM4A Polyclonal antibody

LAPTM4A Polyclonal Antibody for WB, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 30627-1-AP

Print datasheet

Synonyms

HUMORF13, KIAA0108, LAPTM4, LAPTM4A, MBNT, Mtrp

验证数据展示

  • WB analysis using 30627-1-AP

    WB analysis using 30627-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30627-1-AP (LAPTM4A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse kidney using 30627-1-AP

    IF Staining of mouse kidney using 30627-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using LAPTM4A antibody (30627-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse kidney using 30627-1-AP

    IF Staining of mouse kidney using 30627-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using LAPTM4A antibody (30627-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inmouse liver tissue
Positive IF-P detected inmouse kidney tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30627-1-AP targets LAPTM4A in WB, IF-P, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB, IF-P, ELISA Application Description
Tested Reactivity Human, mouse
Immunogen LAPTM4A fusion protein Ag6501 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name lysosomal protein transmembrane 4 alpha
Synonyms HUMORF13, KIAA0108, LAPTM4, LAPTM4A, MBNT, Mtrp
Calculated Molecular Weight 27 kDa
Observed Molecular Weight70 kDa
GenBank Accession NumberBC000421
Gene Symbol LAPTM4A
Gene ID (NCBI) 9741
RRIDAB_3086376
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15012
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LAPTM4A is an intracellular membrane protein that regulates the function of organic cation transporter-2 (OCT2) through direct interaction. LAPTM4A can also form a complex with A4GALT in the Golgi and shows at about 70 kDa.

实验方案

Product Specific Protocols
WB protocol for LAPTM4A antibody 30627-1-APDownload protocol
IF protocol for LAPTM4A antibody 30627-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols