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LARS Monoclonal antibody

LARS Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1E8D4

Cat no : 67940-1-Ig

Print datasheet

Synonyms

FLJ10595, FLJ21788, hr025Cl, HSPC192, KIAA1352, LARS, LARS1, Leucine tRNA ligase, leucyl tRNA synthetase, LEURS, LEUS, LRS, PIG44, RNTLS

验证数据展示

  • WB analysis using 67940-1-Ig

    WB analysis using 67940-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67940-1-Ig (LARS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control.

  • IF Staining of HeLa using 67940-1-Ig

    IF Staining of HeLa using 67940-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using LARS antibody (67940-1-Ig, Clone: 1E8D4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, PC-12 cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, NIH/3T3 cells, 4T1 cells
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy LARS1 Ready-To-Use IHC Kit. LARS1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®555 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67940-1-Ig targets LARS in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen LARS fusion protein Ag15944 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name leucyl-tRNA synthetase
Synonyms FLJ10595, FLJ21788, hr025Cl, HSPC192, KIAA1352, LARS, LARS1, Leucine tRNA ligase, leucyl tRNA synthetase, LEURS, LEUS, LRS, PIG44, RNTLS
Calculated Molecular Weight 1176 aa, 134 kDa
Observed Molecular Weight 135-140 kDa
GenBank Accession NumberBC151214
Gene Symbol LARS
Gene ID (NCBI) 51520
RRIDAB_2918692
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9P2J5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for LARS antibody 67940-1-IgDownload protocol
IF protocol for LARS antibody 67940-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols