验证数据展示
经过测试的应用
| Positive WB detected in | mouse liver tissue, HeLa cells, rat liver tissue, HepG2 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human liver tissue, mouse lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20344-1-AP targets LASS2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | LASS2 fusion protein Ag14151 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | LAG1 homolog, ceramide synthase 2 |
| 别名 | CerS2, L3, LASS2, SP260, TMSG1 |
| 计算分子量 | 380 aa, 45 kDa |
| 观测分子量 | 45 kDa, 37 kDa |
| GenBank蛋白编号 | BC010032 |
| 基因名称 | LASS2 |
| Gene ID (NCBI) | 29956 |
| RRID | AB_2878677 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96G23 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
LASS2, also known as TMSG1, is a novel suppressor of human cancer metastasis. As one member of LASS family, including LASS1-6, LASS2 mRNA is at the highest level of all LASS members, and has the broadest tissue distribution, particularly abundant in the liver, kidney and brain in mice. The biological roles of LASS2 include protection from aging , hepatic INS resistance, and hepatocellular carcinoma (HCC) progression. LASS2 has been correlated with the degree of invasion and recurrence of carcinomas of the prostate, liver, breast and bladder.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for LASS2 antibody 20344-1-AP | Download protocol |
| IHC protocol for LASS2 antibody 20344-1-AP | Download protocol |
| IP protocol for LASS2 antibody 20344-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cardiovasc Res Nogo-A reduces ceramide de novo biosynthesis to protect from heart failure.
| ||
J Cancer LASS2 impairs proliferation of glioma stem cells and migration and invasion of glioma cells mainly via inhibition of EMT and apoptosis promotion.
| ||
Exp Cell Res MicroRNA-98 promotes drug resistance and regulates mitochondrial dynamics by targeting LASS2 in bladder cancer cells.
| ||
Toxicol Mech Methods Chronic ethanol exposure induces cardiac fibroblast transdifferentiation via ceramide accumulation and oxidative stress | ||
J Cancer LASS2 regulates invasion and chemoresistance via ERK/Drp1 modulated mitochondrial dynamics in bladder cancer cells.
| ||
Tumour Biol miR-9 promotes cell proliferation and inhibits apoptosis by targeting LASS2 in bladder cancer. |
