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LASS2 Polyclonal antibody

LASS2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 30183-1-AP

Print datasheet

Synonyms

Tumor metastasis-suppressor gene 1 protein, Sphingosine N-acyltransferase CERS2, EC:2.3.1.297, EC:2.3.1.24, CerS2

验证数据展示

  • WB analysis using 30183-1-AP

    WB analysis using 30183-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30183-1-AP (LASS2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human intrahepatic cholangiocarcinoma using 30183-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 30183-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 30183-1-AP (LASS2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human intrahepatic cholangiocarcinoma using 30183-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 30183-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 30183-1-AP (LASS2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of HeLa using 30183-1-AP

    WB analysis of HeLa using 30183-1-AP

    WB result of LASS2 antibody (30183-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LASS2 transfected HeLa cells.

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, mouse liver tissue, rat liver tissue
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30183-1-AP targets LASS2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen LASS2 fusion protein Ag32769 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name LAG1 homolog, ceramide synthase 2
Synonyms Tumor metastasis-suppressor gene 1 protein, Sphingosine N-acyltransferase CERS2, EC:2.3.1.297, EC:2.3.1.24, CerS2
Calculated Molecular Weight 380 aa, 45 kDa
Observed Molecular Weight37 kDa
GenBank Accession NumberBC010032
Gene Symbol LASS2
Gene ID (NCBI) 29956
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96G23
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LASS2, also known as TMSG1, is a novel suppressor of human cancer metastasis. As one member of LASS family, including LASS1-6, LASS2 mRNA is at the highest level of all LASS members, and has the broadest tissue distribution, particularly abundant in the liver, kidney and brain in mice. The biological roles of LASS2 include protection from aging , hepatic INS resistance, and hepatocellular carcinoma (HCC) progression. LASS2 has been correlated with the degree of invasion and recurrence of carcinomas of the prostate, liver, breast and bladder.

实验方案

Product Specific Protocols
WB protocol for LASS2 antibody 30183-1-APDownload protocol
IHC protocol for LASS2 antibody 30183-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols