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  • Featured Product
  • KD/KO Validated

LATS1 Polyclonal antibody, PBS Only

LATS1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 17049-1-PBS

Print datasheet

Synonyms

WARTS, Serine/threonine-protein kinase LATS1, LATS 1, h-warts, EC:2.7.11.1

验证数据展示

  • WB analysis of HepG2 using 17049-1-AP (same clone as 17049-1-PBS)

    WB analysis of HepG2 using 17049-1-AP (same clone as 17049-1-PBS)

    WB result of LATS1 antibody (17049-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LATS1 transfected HepG2 cells. This data was developed using the same antibody clone with 17049-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 17049-1-AP (same clone as 17049-1-PBS)

    IHC staining of human breast cancer using 17049-1-AP (same clone as 17049-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17049-1-AP (LATS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17049-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 17049-1-AP (same clone as 17049-1-PBS)

    IHC staining of human breast cancer using 17049-1-AP (same clone as 17049-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17049-1-AP (LATS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17049-1-PBS in a different storage buffer formulation.

  • WB analysis using 17049-1-AP (same clone as 17049-1-PBS)

    WB analysis using 17049-1-AP (same clone as 17049-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17049-1-AP (LATS1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17049-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 17049-1-AP (same clone as 17049-1-PBS)

    IF Staining of HeLa using 17049-1-AP (same clone as 17049-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using LATS1 antibody (17049-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 17049-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 17049-1-AP (same clone as 17049-1-PBS)

    IF Staining of HeLa using 17049-1-AP (same clone as 17049-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using LATS1 antibody (17049-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 17049-1-PBS in a different storage buffer formulation.

产品信息

17049-1-PBS targets LATS1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen LATS1 fusion protein Ag10709 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name LATS, large tumor suppressor, homolog 1 (Drosophila)
Synonyms WARTS, Serine/threonine-protein kinase LATS1, LATS 1, h-warts, EC:2.7.11.1
Calculated Molecular Weight 15 kDa, 127 kDa
Observed Molecular Weight 140-150 kDa, 120 kDa
GenBank Accession NumberBC015665
Gene Symbol LATS1
Gene ID (NCBI) 9113
RRIDAB_2281011
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95835
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

LATS1(Large tumor suppressor homolog 1) is also named as WARTS and belongs to the AGC Ser/Thr protein kinase family. The gene encodes a highly conserved (from fly to human) protein kinase that plays a crucial role in the prevention of tumor formation by controlling the progression of mitosis. The expression of both long(170 kDa) and short lats1 isoforms(120 kDa) in vertebrate retinal cells raises the possibility that these lats1 proteins may act as negative key regulators of the cell cycle each of them performing a unique role (PMID:15777619). In mammalian cells, LATS1 was phosphorylated in a cell cycle-dependent manner and complexed with CDC2 in early mitosis (PMID:9988268). LATS1 also can be detected as 120 kDa and 140-150 kDa, and play a key role in the regulation of Hippo pathway (PMID: 27940445).