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验证数据展示

  • WB analysis using 66569-1-Ig (same clone as 66569-1-PBS)

    WB analysis using 66569-1-Ig (same clone as 66569-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66569-1-Ig (LATS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66569-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 66569-1-Ig (same clone as 66569-1-PBS)

    WB analysis of HepG2 using 66569-1-Ig (same clone as 66569-1-PBS)

    WB result of LATS1 antibody (66569-1-Ig; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-LATS1 transfected HepG2 cells. This data was developed using the same antibody clone with 66569-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 66569-1-Ig (same clone as 66569-1-PBS)

    WB analysis of HepG2 using 66569-1-Ig (same clone as 66569-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66569-1-Ig (LATS1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66569-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

LATS1 Monoclonal antibody, PBS Only

LATS1 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 66569-1-PBS

产品说明书

CloneNo. 2A8F1

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

WB, Indirect ELISA

别名

h warts, LATS1, WARTS, WARTS protein kinase, wts

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66569-1-PBS targets LATS1 in WB, Indirect ELISA applications and shows reactivity with Human samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 Human
免疫原 LATS1 fusion protein Ag11140 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 LATS, large tumor suppressor, homolog 1 (Drosophila)
别名 h warts, LATS1, WARTS, WARTS protein kinase, wts
计算分子量 15 kDa, 127 kDa
观测分子量 140-150 kDa, 120 kDa
GenBank蛋白编号BC015665
基因名称 LATS1
Gene ID (NCBI) 9113
RRIDAB_2881930
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO95835
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

LATS1(Large tumor suppressor homolog 1) is also named as WARTS and belongds to the AGC Ser/Thr protein kinase family. The gene encodes a highly conserved (from fly to human) protein kinase that plays a crucial role in the prevention of tumor formation by controlling the progression of mitosis. The expression of both long(170 kDa) and short lats1 isoforms(120 kDa) in vertebrate retinal cells raises the possibility that these lats1 proteins may act as negative key regulators of the cell cycle each of them performing a unique role (PMID:15777619). In mammalian cells, LATS1 was phosphorylated in a cell cycle-dependent manner and complexed with CDC2 in early mitosis (PMID:9988268). LATS1 also can be detected as 120 kDa and 140-150 kDa, and play a key role in the regulation of Hippo pathway (PMID: 27940445).