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LAYN Polyclonal antibody

LAYN Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 20535-1-AP

Print datasheet

Synonyms

UNQ208/PRO234, Layilin

验证数据展示

  • IHC staining of human liver cancer using 20535-1-AP

    IHC staining of human liver cancer using 20535-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20535-1-AP (LAYN antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 20535-1-AP

    WB analysis using 20535-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20535-1-AP (LAYN antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 20535-1-AP

    IF Staining of HepG2 using 20535-1-AP

    Immunofluorescent analysis of HepG2 cells, using LAYN antibody 20535-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

经过测试的应用

Positive WB detected inA549 cells, mouse brain tissue, NCI-H1299 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20535-1-AP targets LAYN in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse
Cited Reactivitymouse
Immunogen LAYN fusion protein Ag14508 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name layilin
Synonyms UNQ208/PRO234, Layilin
Calculated Molecular Weight 382 aa, 43 kDa
Observed Molecular Weight 43 kDa
GenBank Accession NumberBC025407
Gene Symbol LAYN
Gene ID (NCBI) 143903
RRIDAB_10696315
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6UX15
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for LAYN antibody 20535-1-APDownload protocol
IHC protocol for LAYN antibody 20535-1-APDownload protocol
IF protocol for LAYN antibody 20535-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IF

Environ Pollut

Silica nanoparticles suppressed the spermatogenesis via downregulation of miR-450b-3p by targeting Layilin in spermatocyte of mouse

Authors - Guiqing Zhou
View Article