验证数据展示
经过测试的应用
| Positive WB detected in | mouse liver tissue, A431 cells, A549 cells, HeLa cells, Jurkat cells, L02 cells, rat liver tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | L02 cells, HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy LBP Ready-To-Use IHC Kit. LBP primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IHC | See 1 publications below |
产品信息
11836-1-AP targets LBP in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, rat |
| 免疫原 | LBP fusion protein Ag2431 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | lipopolysaccharide binding protein |
| 别名 | Lipopolysaccharide-binding protein |
| 计算分子量 | 481 aa, 53 kDa |
| 观测分子量 | 63-67 kDa |
| GenBank蛋白编号 | BC022256 |
| 基因名称 | LBP |
| Gene ID (NCBI) | 3929 |
| RRID | AB_2097479 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P18428 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
LBP belongs to the BPI/LBP/Plunc superfamily and BPI/LBP family. It binds to the lipid A moiety of bacterial lipopolysaccharides (LPS) which is a glycolipid present in the outer membrane of all Gram-negative bacteria. The LBP/LPS complex seems to interact with the CD14 receptor. LBP is involved in the acute-phase immunologic response to gram-negative bacterial infections. In many tissue/cells, we get 53-60 kDa and 63-67 kDa(Glycosylation form).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for LBP antibody 11836-1-AP | Download protocol |
| IHC protocol for LBP antibody 11836-1-AP | Download protocol |
| IF protocol for LBP antibody 11836-1-AP | Download protocol |
| IP protocol for LBP antibody 11836-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Neurosurg Association of decreased levels of lipopolysaccharide-binding protein with OKN-007-induced regression of tumor growth in an F98 rat glioma model. | ||
Cancer Sci Circulating exosomes contain protein biomarkers of metastatic non-small-cell lung cancer. | ||
J Biomed Mater Res A The characteristic regulation of gene expression Lbp and Sod3 in peri-implant connective tissue of rats. |
