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验证数据展示

  • WB analysis using 30222-1-AP

    WB analysis using 30222-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30222-1-AP (LCMT1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse uterus using 30222-1-AP

    IHC staining of mouse uterus using 30222-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse uterus tissue slide using 30222-1-AP (LCMT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

LCMT1 Polyclonal antibody

LCMT1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 30222-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

WB, IHC, ELISA

Synonyms

CGI 68, LCMT, LCMT1, PPMT1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, HEK-293 cells, HeLa cells, HepG2 cellls, MCF-7 cells, U-87 MG cells
Positive IHC detected inmouse uterus tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30222-1-AP targets LCMT1 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human, Mouse
Immunogen LCMT1 fusion protein Ag32038 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name leucine carboxyl methyltransferase 1
Synonyms CGI 68, LCMT, LCMT1, PPMT1
Calculated Molecular Weight 38 kDa
Observed Molecular Weight 38-41 kDa
GenBank Accession NumberBC001214
Gene Symbol LCMT1
Gene ID (NCBI) 51451
RRIDAB_2935534
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UIC8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Leucine carboxyl methyltransferase 1 (LCMT1) belongs to the methyltransferase superfamily (LCMT family). It methylates the carboxyl group of the C-terminal leucine residue of protein phosphatase 2A catalytic subunits to form alpha-leucine ester residues. LCMT1 has 3 isoforms with the MW of 38 kDa, 41 kDa, and 32 kDa.

实验方案

Product Specific Protocols
WB protocol for LCMT1 antibody 30222-1-APDownload protocol
IHC protocol for LCMT1 antibody 30222-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols