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NGAL Polyclonal antibody
NGAL Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HT-29 cells |
Positive IHC detected in | human colon cancer tissue, Human bowens disease Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | RAW 264.7 cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
30700-1-AP targets NGAL in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
Tested Reactivity | human, mouse |
Immunogen | NGAL fusion protein Ag33369 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | lipocalin 2 |
Synonyms | lipocalin 2, LCN2, Lcn 2, HNL, 25 kDa alpha-2-microglobulin-related subunit of MMP-9 |
Observed Molecular Weight | 22-25 kDa |
Gene Symbol | NGAL/LCN2 |
Gene ID (NCBI) | 3934 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P80188 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for NGAL antibody 30700-1-AP | Download protocol |
IHC protocol for NGAL antibody 30700-1-AP | Download protocol |
IF protocol for NGAL antibody 30700-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |