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  • Featured Product
  • KD/KO Validated

NGAL Polyclonal antibody

NGAL Polyclonal Antibody for WB, IF/ICC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IF/ICC, IHC, ELISA

Conjugate

Unconjugated

Cat No : 30700-1-AP

Print datasheet

Synonyms

24p3, HNL, LCN2, lipocalin 2, NGAL, Oncogene 24p3, p25

验证数据展示

  • WB analysis using 30700-1-AP

    WB analysis using 30700-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30700-1-AP (Lipocalin-2/NGAL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HT-29 using 30700-1-AP

    WB analysis of HT-29 using 30700-1-AP

    WB result of NGAL antibody (30700-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NGAL transfected HT-29 cells.

  • IHC staining of human colon cancer using 30700-1-AP

    IHC staining of human colon cancer using 30700-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 30700-1-AP (Lipocalin-2/NGAL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NGAL/LCN2 antibody included
  • IF Staining of RAW 264.7 using 30700-1-AP

    IF Staining of RAW 264.7 using 30700-1-AP

    Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using Lipocalin-2/NGAL antibody (30700-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inHT-29 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inRAW 264.7 cells
Planning an IHC experiment? We recommend our IHCeasy NGAL/LCN2 Ready-To-Use IHC Kit. NGAL/LCN2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30700-1-AP targets NGAL in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB, IF/ICC, IHC, ELISA Application Description
Tested Reactivity Human, mouse
Immunogen NGAL fusion protein Ag33369 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name lipocalin 2
Synonyms 24p3, HNL, LCN2, lipocalin 2, NGAL, Oncogene 24p3, p25
Observed Molecular Weight22-25 kDa
Gene Symbol LCN2
Gene ID (NCBI) 3934
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP80188
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Lipocalin-2 (LCN-2) is a adipocytokine also referred to as neutrophil gelatinase-associated lipocalin (NGAL). Lipocalin-2 is a circulatory protein responsible for the transportation of small and hydrophobic molecules to target organs. Lipocalin-2 is used as a biomarker for acute and chronic renal injury. It is present in a large variety of cells including neutrophil, hepatocytes, lung, bone marrow, adipose tissue, macrophages, thymus, non-neoplastic breast duct, prostate, and renal cells. Different functions have been associated with Lipocalin-2, including antibacterial, anti-inflammatory, and protection against cell and tissue stress (PMID:34463264).

实验方案

Product Specific Protocols
WB protocol for NGAL antibody 30700-1-APDownload protocol
IHC protocol for NGAL antibody 30700-1-APDownload protocol
IF protocol for NGAL antibody 30700-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols