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验证数据展示

  • WB analysis using 30700-1-AP

    WB analysis using 30700-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30700-1-AP (Lipocalin-2/NGAL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HT-29 using 30700-1-AP

    WB analysis of HT-29 using 30700-1-AP

    WB result of NGAL antibody (30700-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NGAL transfected HT-29 cells.

  • IHC staining of human colon cancer using 30700-1-AP

    IHC staining of human colon cancer using 30700-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 30700-1-AP (Lipocalin-2/NGAL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NGAL/LCN2 antibody included
  • IHC staining of Human bowens disease using 30700-1-AP

    IHC staining of Human bowens disease using 30700-1-AP

    Immunohistochemical analysis of paraffin-embedded human brown disease slide using 30700-1-AP (NGAL antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NGAL/LCN2 antibody included
  • IF Staining of RAW 264.7 using 30700-1-AP

    IF Staining of RAW 264.7 using 30700-1-AP

    Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using Lipocalin-2/NGAL antibody (30700-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Featured Product
  • KD/KO验证

Lipocalin-2/NGAL Polyclonal antibody

Lipocalin-2/NGAL Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 30700-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

LCN2, lipocalin 2, NGAL, 24p3, 25 kDa alpha-2-microglobulin-related subunit of MMP-9

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHT-29 cells
Positive IHC detected inhuman colon cancer tissue, Human bowens disease
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inRAW 264.7 cells
Planning an IHC experiment? We recommend our IHCeasy NGAL/LCN2 Ready-To-Use IHC Kit. NGAL/LCN2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30700-1-AP targets Lipocalin-2/NGAL in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 Lipocalin-2/NGAL fusion protein Ag33369 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 lipocalin 2
别名 LCN2, lipocalin 2, NGAL, 24p3, 25 kDa alpha-2-microglobulin-related subunit of MMP-9
观测分子量22-25 kDa
基因名称 NGAL/LCN2
Gene ID (NCBI) 3934
RRIDAB_3086393
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP80188
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Lipocalin-2 (LCN-2) is a adipocytokine also referred to as neutrophil gelatinase-associated lipocalin (NGAL). Lipocalin-2 is a circulatory protein responsible for the transportation of small and hydrophobic molecules to target organs. Lipocalin-2 is used as a biomarker for acute and chronic renal injury. It is present in a large variety of cells including neutrophil, hepatocytes, lung, bone marrow, adipose tissue, macrophages, thymus, non-neoplastic breast duct, prostate, and renal cells. Different functions have been associated with Lipocalin-2, including antibacterial, anti-inflammatory, and protection against cell and tissue stress (PMID:34463264).

实验方案

Product Specific Protocols
WB protocol for Lipocalin-2/NGAL antibody 30700-1-APDownload protocol
IHC protocol for Lipocalin-2/NGAL antibody 30700-1-APDownload protocol
IF protocol for Lipocalin-2/NGAL antibody 30700-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols