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LCOR Polyclonal antibody

LCOR Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 14476-1-AP

Print datasheet

Synonyms

MLR2, Mblk1-related protein 2, Ligand-dependent corepressor, C10orf12

验证数据展示

  • WB analysis of HeLa using 14476-1-AP

    WB analysis of HeLa using 14476-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14476-1-AP (LCOR antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human endometrial cancer using 14476-1-AP

    IHC staining of human endometrial cancer using 14476-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 14476-1-AP (LCOR antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human endometrial cancer using 14476-1-AP

    IHC staining of human endometrial cancer using 14476-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 14476-1-AP (LCOR antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HeLa using 14476-1-AP

    IF Staining of HeLa using 14476-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 14476-1-AP (LCOR antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells
Positive IHC detected inhuman endometrial cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14476-1-AP targets LCOR in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen LCOR fusion protein Ag5851 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ligand dependent nuclear receptor corepressor
Synonyms MLR2, Mblk1-related protein 2, Ligand-dependent corepressor, C10orf12
Calculated Molecular Weight 47 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC053359
Gene Symbol LCOR
Gene ID (NCBI) 84458
RRIDAB_2136862
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96JN0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LCOR is a transcriptional corepressor widely expressed in fetal and adult tissues that is recruited to agonist-bound nuclear receptors through a single LxxLL motif, also referred to as a nuclear receptor (NR) box [PMID:12535528]. It functions through recruitment of C-terminal binding proteins (CtBPs) and histone deacetylases (HDACs) to its N-terminal and central domains, respectively [PMID:22277651]. It is a scaffold for both histone deacetylases (HDACs) and C-terminal binding protein (CtBP) corepressors and functions by both HDAC-dependent and -independent mechanisms in a receptor-specific manner[PMID:19744932].

实验方案

Product Specific Protocols
WB protocol for LCOR antibody 14476-1-APDownload protocol
IHC protocol for LCOR antibody 14476-1-APDownload protocol
IF protocol for LCOR antibody 14476-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols